Combined hydrodynamic mixer and gas dynamic virtual nozzle devices for serial femtosecond crystallography

Gerrit Brehm, Austin Echelmeier, Jesse Coe, Chelsie E. Conrad, Garrett Nelson, Jorvani Cruz Villarreal, Uwe Weierstall, John Spence, Sarah Köster, Petra Fromme, Alexandra Ros

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

With the advent of X-ray free electron lasers (XFELs), time-resolved serial femtosecond X-ray crystallography (TR-SFX) studies have become possible elucidating insight in protein dynamics of important processes such as photosynthesis or antibiotic resistance. A major factor limiting TR-SFX experiments are reliable injection technologies compatible with the liquid jet injection methods employed for SFX which allow fast mixing and reach suitable reaction time points. Here, we present the first 3D printed hybrid nozzle device injecting aqueous protein crystal jets after fast mixing (<5 ms), reaching reaction time points (~150 ms) suitable to resolve enzyme kinetics.

Original languageEnglish (US)
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages1495-1496
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - Jan 1 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • 3D printing
  • Mixing
  • Serial femtosecond crystallography
  • Time-resolved
  • X-ray free electron laser

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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