@inproceedings{64705ab841c9488ab8827512015ee37d,
title = "Combined hydrodynamic mixer and gas dynamic virtual nozzle devices for serial femtosecond crystallography",
abstract = "With the advent of X-ray free electron lasers (XFELs), time-resolved serial femtosecond X-ray crystallography (TR-SFX) studies have become possible elucidating insight in protein dynamics of important processes such as photosynthesis or antibiotic resistance. A major factor limiting TR-SFX experiments are reliable injection technologies compatible with the liquid jet injection methods employed for SFX which allow fast mixing and reach suitable reaction time points. Here, we present the first 3D printed hybrid nozzle device injecting aqueous protein crystal jets after fast mixing (<5 ms), reaching reaction time points (~150 ms) suitable to resolve enzyme kinetics.",
keywords = "3D printing, Mixing, Serial femtosecond crystallography, Time-resolved, X-ray free electron laser",
author = "Gerrit Brehm and Austin Echelmeier and Jesse Coe and Conrad, {Chelsie E.} and Garrett Nelson and Villarreal, {Jorvani Cruz} and Uwe Weierstall and John Spence and Sarah K{\"o}ster and Petra Fromme and Alexandra Ros",
year = "2020",
month = jan,
day = "1",
language = "English (US)",
series = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",
publisher = "Chemical and Biological Microsystems Society",
pages = "1495--1496",
booktitle = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017",
note = "21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 ; Conference date: 22-10-2017 Through 26-10-2017",
}