Coinfections of novel polyomavirus, anelloviruses and a recombinant strain of myxoma virus-MYXV-tol identified in Iberian hares

Ana Ägueda-Pinto, Simona Kraberger, Michael C. Lund, Christian Gortázar, Grant McFadden, Arvind Varsani, Pedro J. Esteves

Research output: Contribution to journalArticle

Abstract

Viruses are ubiquitous in nature; however, very few have been identified in the Leporid species. In the fall of 2018, an outbreak of myxomatosis in Iberian hares (Lepus granatensis) was reported in Spain and a novel recombinant myxoma virus strain (MYXV-Tol) was identified. To investigate variability within the recombinant region of the MYXV-Tol and identify any potential viral coinfections, samples (ear, eyelid or vaginal) of Iberian hares were collected from Spain and analyzed. The presence of the recombinant region of the MYXV-Tol was confirmed in six out of eleven samples analyzed. Additionally, a polyomavirus (family Polyomaviridae), representing a putative new species, and anelloviruses (family Anelloviridae) belonging to two putative species were identified, some as coinfection with the recombinant MYXV-Tol. The two polyomavirus genomes were identified in two hares and share >99% genome-wide identity. Based on the analysis of their large T-antigen, the new polyomavirus clusters in a distant clade from other mammals sharing <64% amino acid identity. A total of 14 anelloviruses were identified, which share 63-99% genome-wide identity. Overall, our results show a coinfection of different DNA viruses in the studied samples and raise awareness regarding the extensive unsampled diversity of viruses in hares.

Original languageEnglish (US)
Article number340
JournalViruses
Volume12
Issue number3
DOIs
StatePublished - Jan 1 2020

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Keywords

  • Anelloviridae
  • Coinfection
  • Iberian hare
  • Leporidae
  • Lepus granatensis
  • Myxoma virus
  • Polyomaviridae
  • Spain

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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