Coherent convergent-beam time-resolved X-ray diffraction

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    The use of coherent X-ray lasers for structural biology allows the use of nanometre diameter X-ray beams with large beam divergence. Their application to the structure analysis of protein nanocrystals and single particles raises new challenges and opportunities. We discuss the form of these coherent convergent-beam (CCB) hard X-ray diffraction patterns and their potential use for time-resolved crystallography, normally achieved by Laue (polychromatic) diffraction, for which the monochromatic laser radiation of a free-electron X-ray laser is unsuitable. We discuss the possibility of obtaining single-shot, angle-integrated rocking curves from CCB patterns, and the dependence of the resulting patterns on the focused beam coordinate when the beam diameter is larger or smaller than a nanocrystal, or smaller than one unit cell. We show how structure factor phase information is provided at overlapping interfering orders and how a common phase origin between different shots may be obtained. Their use in refinement of the phase-sensitive intensity between overlapping orders is suggested.

    Original languageEnglish (US)
    Article number20130325
    JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
    Volume369
    Issue number1647
    DOIs
    StatePublished - Jul 17 2014

    Fingerprint

    X ray lasers
    nanocrystals
    X-ray diffraction
    X-Ray Diffraction
    Nanocrystals
    lasers
    X-radiation
    Lasers
    laser
    X-Rays
    X ray diffraction
    Nanoparticles
    Crystallography
    Free electron lasers
    Laser radiation
    crystallography
    Diffraction patterns
    Diffraction
    X rays
    diffraction

    Keywords

    • Coherent convergent beam
    • Coherent nanodiffraction
    • Free-electron X-ray laser
    • Phase determination
    • Time-resolved diffraction

    ASJC Scopus subject areas

    • Agricultural and Biological Sciences(all)
    • Biochemistry, Genetics and Molecular Biology(all)
    • Medicine(all)

    Cite this

    Coherent convergent-beam time-resolved X-ray diffraction. / Spence, John; Zatsepin, Nadia; Li, Chufeng.

    In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, No. 1647, 20130325, 17.07.2014.

    Research output: Contribution to journalArticle

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