TY - JOUR
T1 - Cochleate lipid cylinders
T2 - formation by fusion of unilamellar lipid vesicles
AU - Papahadjopoulos, D.
AU - Vail, W. J.
AU - Jacobson, K.
AU - Poste, G.
N1 - Funding Information:
capture of large macromolecules and large radius of curvature are important requirements. This work was supported by a grant from the National Institute of Health (GM-18921) and the Multiple Sclerosis Society of Canada. We thank Mr T. Isac and Mrs R. Lazo for expert technical assistance, and Drs S. Nir and S. Hui for stimulating discussions.
PY - 1975/7/3
Y1 - 1975/7/3
N2 - Freeze-fracture electron microscopy was used to study the morphological changes occurring following the addition of Ca2+ to sonicated preparations of phosphatidylserine in aqueous NaCl buffer. Before the addition of Ca2+, preparations contained only small (200-500 Å diameter) spheroidal vesicles. After the addition of Ca2+ (10 mM) and incubation for 1 h at 37°C preparations contained only large (2000-10 000 Å) apparently multilamellar structures many of which were cylindrical in shape. The lamellae in these cylinders appear to be folded in a spiral configuration. Addition of EDTA to these preparations produced large, closed, spherical, unilamellar vesicles. We suggest the name cochleate lipid cylinders for the spiral structures and propose that they are formed by fusion of unilamellar vesicles into large sheets which fold spirally to form cylinders.
AB - Freeze-fracture electron microscopy was used to study the morphological changes occurring following the addition of Ca2+ to sonicated preparations of phosphatidylserine in aqueous NaCl buffer. Before the addition of Ca2+, preparations contained only small (200-500 Å diameter) spheroidal vesicles. After the addition of Ca2+ (10 mM) and incubation for 1 h at 37°C preparations contained only large (2000-10 000 Å) apparently multilamellar structures many of which were cylindrical in shape. The lamellae in these cylinders appear to be folded in a spiral configuration. Addition of EDTA to these preparations produced large, closed, spherical, unilamellar vesicles. We suggest the name cochleate lipid cylinders for the spiral structures and propose that they are formed by fusion of unilamellar vesicles into large sheets which fold spirally to form cylinders.
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U2 - 10.1016/0005-2736(75)90299-0
DO - 10.1016/0005-2736(75)90299-0
M3 - Article
C2 - 805602
AN - SCOPUS:0016820946
VL - 394
SP - 483
EP - 491
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 3
ER -