TY - JOUR
T1 - Cochleate lipid cylinders
T2 - formation by fusion of unilamellar lipid vesicles
AU - Papahadjopoulos, D.
AU - Vail, W. J.
AU - Jacobson, K.
AU - Poste, G.
PY - 1975/7/3
Y1 - 1975/7/3
N2 - Freeze-fracture electron microscopy was used to study the morphological changes occurring following the addition of Ca2+ to sonicated preparations of phosphatidylserine in aqueous NaCl buffer. Before the addition of Ca2+, preparations contained only small (200-500 Å diameter) spheroidal vesicles. After the addition of Ca2+ (10 mM) and incubation for 1 h at 37°C preparations contained only large (2000-10 000 Å) apparently multilamellar structures many of which were cylindrical in shape. The lamellae in these cylinders appear to be folded in a spiral configuration. Addition of EDTA to these preparations produced large, closed, spherical, unilamellar vesicles. We suggest the name cochleate lipid cylinders for the spiral structures and propose that they are formed by fusion of unilamellar vesicles into large sheets which fold spirally to form cylinders.
AB - Freeze-fracture electron microscopy was used to study the morphological changes occurring following the addition of Ca2+ to sonicated preparations of phosphatidylserine in aqueous NaCl buffer. Before the addition of Ca2+, preparations contained only small (200-500 Å diameter) spheroidal vesicles. After the addition of Ca2+ (10 mM) and incubation for 1 h at 37°C preparations contained only large (2000-10 000 Å) apparently multilamellar structures many of which were cylindrical in shape. The lamellae in these cylinders appear to be folded in a spiral configuration. Addition of EDTA to these preparations produced large, closed, spherical, unilamellar vesicles. We suggest the name cochleate lipid cylinders for the spiral structures and propose that they are formed by fusion of unilamellar vesicles into large sheets which fold spirally to form cylinders.
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U2 - 10.1016/0005-2736(75)90299-0
DO - 10.1016/0005-2736(75)90299-0
M3 - Article
C2 - 805602
AN - SCOPUS:0016820946
VL - 394
SP - 483
EP - 491
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
SN - 0005-2736
IS - 3
ER -