Coccidioides immitis Antigen 2: Analysis of gene and protein

Yufan Zhu, Chunmu Yang, D. Mitchell Magee, Rebecca A. Cox

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Antigen 2 is a glycosylated protein present in the cell walls of the dimorphic fungus Coccidioides immitis. Using oligodeoxyribonucleotide (oligo) primers based on the sequences of Ag2 cDNA, the gene encoding Ag2 was cloned from genomic DNA derived from the mycelial phase of C. immitis by PCR. Nucleotide (nt) sequence analyses showed a 582 base pair (bp) ORF disrupted by two introns which are 78 bp and 101 bp long. The deduced primary translation product consists of 194 amino acids (aa), contains an N-terminal putative signal sequence to allow transport into the endoplasmic reticulum, and a C-terminal putative signal sequence to enable a GPI anchor addition. Putative GPI anchor/cleavage site and O-glycosylation sites, as well as phosphorylation and myristoylation sites are also present. On the basis of these analyses, we predict that a prepro-Ag2 undergoes a post-translational modification to yield the mature glycosylated Ag2 protein which is anchored on the extracellular plasma membrane of mycelial and spherule-phase cells.

Original languageEnglish (US)
Pages (from-to)121-125
Number of pages5
JournalGene
Volume181
Issue number1-2
DOIs
StatePublished - Nov 28 1996
Externally publishedYes

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Keywords

  • Fungal intron
  • Pathogenic fungus
  • Recombinant DNA
  • T cell-reactive antigen
  • Tetrapeptide repeat

ASJC Scopus subject areas

  • Genetics

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