Co-induction of lymphokine synthesis by the antineoplastic bryostatins

H. Mohr, George Pettit, A. Plessing-Menze

Research output: Contribution to journalArticle

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Abstract

The macrocyclic lactone bryostatins, isolated from marine bryozoans, have been found to be strong inhibitors of e.g., the P 388 murine lymphocyte leukemia cell line and in vivo systems. Presently, bryostatin 1 is under preclinical development as a potential anticancer drug, although the bryostatins exhibit some of the same biological effects as the tumor promoting phorbol-12-myristate-13-acetate (PMA), especially activation of protein kinase C in certain cell types. In our experiments, we investigated the influence of bryostatin 1 on the synthesis of interleukin 2 (IL2) and interferon-γ (IFN-γ) by ionophore A23187 or mitogen-induced human blood lymphocytes. These results were then compared with those achieved using the two tumor promoters PMA and teleocidin. Our data indicate that bryostatin 1 is comparable to these two other drugs in increasing production of the two lymphokines 10-100-fold. The IL2 and IFN-γ-production kinetics of cultures induced with either A23187/bryostatin 1 or A23187/PMA were practically identical. The general pattern of peptides, however, released from bryostatin 1 coinduced cultures differed from that obtained when PMA was used.

Original languageEnglish (US)
Pages (from-to)420-430
Number of pages11
JournalImmunobiology
Volume175
Issue number5
StatePublished - 1987

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Bryostatins
Lymphokines
Antineoplastic Agents
Calcimycin
Acetates
Interferons
Interleukin-2
Lymphocytes
Ionophores
Lactones
Mitogens
Pharmaceutical Preparations
Carcinogens
Protein Kinase C
Leukemia
bryostatin 1
Cell Line
Peptides
phorbol-12-myristate
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Co-induction of lymphokine synthesis by the antineoplastic bryostatins. / Mohr, H.; Pettit, George; Plessing-Menze, A.

In: Immunobiology, Vol. 175, No. 5, 1987, p. 420-430.

Research output: Contribution to journalArticle

Mohr, H, Pettit, G & Plessing-Menze, A 1987, 'Co-induction of lymphokine synthesis by the antineoplastic bryostatins', Immunobiology, vol. 175, no. 5, pp. 420-430.
Mohr, H. ; Pettit, George ; Plessing-Menze, A. / Co-induction of lymphokine synthesis by the antineoplastic bryostatins. In: Immunobiology. 1987 ; Vol. 175, No. 5. pp. 420-430.
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