Cloning of the vaccinia virus telomere in a yeast plasmid vector

Aloysius M. DeLange, Bruce Futcher, Richard Morgan, Grant McFadden

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Abstract

The vaccinia virus DNA telomere, which contains a covalently closed hairpin structure, has been cloned in a yeast plasmid vector. Restriction mapping indicates that the cloned vaccinia telomere is maintained in yeast not in its native hairpin configuration but as an inverted repeat structure, within a circular plasmid, with the sequences of the viral hairpin now at the axis of symmetry of an imperfect palindrome. As such, the cloned telomere resembles the telomeric replicative intermediate observed during vaccinia virus DNA replication. Small deletions and duplications in the viral inverted repeats of different clones suggest a model in which the observed circular plasmids were generated in yeast by the replication of hybrid linear DNA molecules consisting of the linearized yeast vector flanked by two hairpin-containing vaccinia termini.

Original languageEnglish (US)
Pages (from-to)13-21
Number of pages9
JournalGene
Volume27
Issue number1
DOIs
StatePublished - Jan 1984

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Keywords

  • Hairpin termini
  • palindrome
  • recombinant DNA
  • replicative intermediate

ASJC Scopus subject areas

  • Genetics

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