Cloning of the Human Dopamine D5 Receptor Gene and Identification of a Highly Polymorphic Microsatellite for the DRD5 Locus That Shows Tight Linkage to the Chromosome 4p Reference Marker RAF1P1

Robin Sherrington, Baljinder Mankoo, John Attwood, Gursharon Kalsi, David Curtis, Kenneth Buetow, Sue Povey, Hugh Gurling

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

We identified a cosmid clone with exact sequence homology to part of the human dopamine D5 receptor gene (DRD5) after screening a cosmid library with the human DRD1 gene. The dopamine D5 receptor was mapped to chromosome 4p15.1-p15.3 by in situ hybridization and using a somatic cell hybrid panel. We report here the further localization of the DRD5 gene following identification of a highly polymorphic dinucleotide repeat sequence in the cosmid clone. The microsatellite (D5(CT/GT/GA)n) had 12 alleles with a polymorphic information content value of 0.77. Linkage analysis in 39 CEPH pedigrees demonstrated tight linkage to the chromosome 4p reference marker RAF1P1 (Zmaxt 20.66 at θf 0.05 and ZmaxM 16.57 at θm 0.07).

Original languageEnglish (US)
Pages (from-to)423-425
Number of pages3
JournalGenomics
Volume18
Issue number2
DOIs
StatePublished - Nov 1993
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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