Cloning and sequencing of the cytoplasmic precursor to the alpha subunit of rat liver mitochondrial succinyl-CoA synthetase.

W. D. Henning, C. Upton, Douglas McFadden, R. Majumdar, W. A. Bridger

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Succinyl-CoA synthetase [succinate-CoA ligase (GDP-forming); EC 6.2.1.4] of rat liver, an alpha beta dimer, is a component of the enzymology of the tricarboxylic acid cycle and functions within the mitochondrial matrix. We have isolated and determined the sequence of a cDNA clone containing the coding sequence of the cytoplasmic precursor to the alpha subunit of this enzyme together with stretches of nontranslated sequence at the 5' and 3' ends. The translated amino acid sequence indicates the presence of a 27-residue N-terminal signal sequence for mitochondrial targeting. The amino acid sequence of the mature alpha subunit shows an extraordinary degree of homology to the alpha subunit of Escherichia coli succinyl-CoA synthetase, with greater than 70% of the residues identical. This suggests that the fundamental differences in the quaternary structures and catalytic functions of the mammalian and bacterial enzymes must be attributable to differences in the beta subunits. mRNA that hybridizes to the cloned DNA is approximately equal to 1800 nucleotide residues in length, confirming that each of the two subunits is encoded separately and does not arise by proteolysis of a primary gene product containing both subunits of the mature protein.

Original languageEnglish (US)
Pages (from-to)1432-1436
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number5
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

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Succinate-CoA Ligases
Organism Cloning
Amino Acid Sequence
Liver
Citric Acid Cycle
Protein Subunits
Enzymes
Protein Sorting Signals
Proteolysis
Nucleotides
Complementary DNA
Clone Cells
Escherichia coli
Messenger RNA
DNA
Genes

ASJC Scopus subject areas

  • General

Cite this

Cloning and sequencing of the cytoplasmic precursor to the alpha subunit of rat liver mitochondrial succinyl-CoA synthetase. / Henning, W. D.; Upton, C.; McFadden, Douglas; Majumdar, R.; Bridger, W. A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 5, 01.01.1988, p. 1432-1436.

Research output: Contribution to journalArticle

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