Clog-free translocation of long DNA in nanofluidic pillar arrays and 30 nm wide channels: A fabrication and hydrodynamic study

Chao Wang, Robert L. Bruce, Elizabeth A. Duch, Jyotica V. Patel, Joshua T. Smith, Yann Astier, Evan G. Colgan, Qinghuang Lin, Gustavo Stolovitzky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We fabricate nanofluidic devices, comprising diamond-shaped nanopillars and nanochannels as narrow as 30 nm using photolithographic techniques, and demonstrate successful translocation of long 1DNA (48.5 kbp) and T4 DNA (166 kbp) through these nanostructures. λ-DNA molecules can transit through 10 μm-long nanochannels in ∼50 ± 10 msec at ∼210 μm/sec without clogging, due to prestretching of the DNA molecules that results from geometrical confinement and straddling of the molecules around the nano-pillars. λ-DNA translocation speed can be linearly tuned from ∼300 to ∼900 μm/sec by electrophoresis with a mobility of (1.3 ± 0.15) × 10-4 cm2/(V · sec). Importantly, T4 DNA molecules translocated through channels as small as 30 nm, extending their length to 73.5 μm in the process, i.e. ∼100 % of its dyed contour length.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1347-1349
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Keywords

  • Dna stretching
  • Nanofluidic channels
  • Nanopillars
  • Single molecule imaging
  • Translocation

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Wang, C., Bruce, R. L., Duch, E. A., Patel, J. V., Smith, J. T., Astier, Y., Colgan, E. G., Lin, Q., & Stolovitzky, G. (2014). Clog-free translocation of long DNA in nanofluidic pillar arrays and 30 nm wide channels: A fabrication and hydrodynamic study. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1347-1349). Chemical and Biological Microsystems Society.