Clog-free translocation of long DNA in nanofluidic pillar arrays and 30 nm wide channels

A fabrication and hydrodynamic study

Chao Wang, Robert L. Bruce, Elizabeth A. Duch, Jyotica V. Patel, Joshua T. Smith, Yann Astier, Evan G. Colgan, Qinghuang Lin, Gustavo Stolovitzky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We fabricate nanofluidic devices, comprising diamond-shaped nanopillars and nanochannels as narrow as 30 nm using photolithographic techniques, and demonstrate successful translocation of long 1DNA (48.5 kbp) and T4 DNA (166 kbp) through these nanostructures. λ-DNA molecules can transit through 10 μm-long nanochannels in ∼50 ± 10 msec at ∼210 μm/sec without clogging, due to prestretching of the DNA molecules that results from geometrical confinement and straddling of the molecules around the nano-pillars. λ-DNA translocation speed can be linearly tuned from ∼300 to ∼900 μm/sec by electrophoresis with a mobility of (1.3 ± 0.15) × 10-4 cm2/(V · sec). Importantly, T4 DNA molecules translocated through channels as small as 30 nm, extending their length to 73.5 μm in the process, i.e. ∼100 % of its dyed contour length.

Original languageEnglish (US)
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages1347-1349
Number of pages3
ISBN (Print)9780979806476
StatePublished - 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Fingerprint

Nanofluidics
DNA
Hydrodynamics
Fabrication
Molecules
Electrophoresis
Nanostructures
Diamonds

Keywords

  • Dna stretching
  • Nanofluidic channels
  • Nanopillars
  • Single molecule imaging
  • Translocation

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Wang, C., Bruce, R. L., Duch, E. A., Patel, J. V., Smith, J. T., Astier, Y., ... Stolovitzky, G. (2014). Clog-free translocation of long DNA in nanofluidic pillar arrays and 30 nm wide channels: A fabrication and hydrodynamic study. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 1347-1349). Chemical and Biological Microsystems Society.

Clog-free translocation of long DNA in nanofluidic pillar arrays and 30 nm wide channels : A fabrication and hydrodynamic study. / Wang, Chao; Bruce, Robert L.; Duch, Elizabeth A.; Patel, Jyotica V.; Smith, Joshua T.; Astier, Yann; Colgan, Evan G.; Lin, Qinghuang; Stolovitzky, Gustavo.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 1347-1349.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wang, C, Bruce, RL, Duch, EA, Patel, JV, Smith, JT, Astier, Y, Colgan, EG, Lin, Q & Stolovitzky, G 2014, Clog-free translocation of long DNA in nanofluidic pillar arrays and 30 nm wide channels: A fabrication and hydrodynamic study. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 1347-1349, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.
Wang C, Bruce RL, Duch EA, Patel JV, Smith JT, Astier Y et al. Clog-free translocation of long DNA in nanofluidic pillar arrays and 30 nm wide channels: A fabrication and hydrodynamic study. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 1347-1349
Wang, Chao ; Bruce, Robert L. ; Duch, Elizabeth A. ; Patel, Jyotica V. ; Smith, Joshua T. ; Astier, Yann ; Colgan, Evan G. ; Lin, Qinghuang ; Stolovitzky, Gustavo. / Clog-free translocation of long DNA in nanofluidic pillar arrays and 30 nm wide channels : A fabrication and hydrodynamic study. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 1347-1349
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AU - Smith, Joshua T.

AU - Astier, Yann

AU - Colgan, Evan G.

AU - Lin, Qinghuang

AU - Stolovitzky, Gustavo

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N2 - We fabricate nanofluidic devices, comprising diamond-shaped nanopillars and nanochannels as narrow as 30 nm using photolithographic techniques, and demonstrate successful translocation of long 1DNA (48.5 kbp) and T4 DNA (166 kbp) through these nanostructures. λ-DNA molecules can transit through 10 μm-long nanochannels in ∼50 ± 10 msec at ∼210 μm/sec without clogging, due to prestretching of the DNA molecules that results from geometrical confinement and straddling of the molecules around the nano-pillars. λ-DNA translocation speed can be linearly tuned from ∼300 to ∼900 μm/sec by electrophoresis with a mobility of (1.3 ± 0.15) × 10-4 cm2/(V · sec). Importantly, T4 DNA molecules translocated through channels as small as 30 nm, extending their length to 73.5 μm in the process, i.e. ∼100 % of its dyed contour length.

AB - We fabricate nanofluidic devices, comprising diamond-shaped nanopillars and nanochannels as narrow as 30 nm using photolithographic techniques, and demonstrate successful translocation of long 1DNA (48.5 kbp) and T4 DNA (166 kbp) through these nanostructures. λ-DNA molecules can transit through 10 μm-long nanochannels in ∼50 ± 10 msec at ∼210 μm/sec without clogging, due to prestretching of the DNA molecules that results from geometrical confinement and straddling of the molecules around the nano-pillars. λ-DNA translocation speed can be linearly tuned from ∼300 to ∼900 μm/sec by electrophoresis with a mobility of (1.3 ± 0.15) × 10-4 cm2/(V · sec). Importantly, T4 DNA molecules translocated through channels as small as 30 nm, extending their length to 73.5 μm in the process, i.e. ∼100 % of its dyed contour length.

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