Clathrin assembly protein AP-2 is detected in both neurons and glia, and its reduction is prominent in layer II of frontal cortex in Alzheimer's disease

Pamela J. Yao; Jill M. Weimer; Timothy M. O’Herron; Paul D. Coleman

doi:10.1016/S0197-4580(00)00228-1

Clathrin assembly protein AP-2 is detected in both neurons and glia, and its reduction is prominent in layer II of frontal cortex in Alzheimer's disease

Pamela J. Yao, Jill M. Weimer, Timothy M. O’Herron, Paul D. Coleman

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

There are several adaptor proteins associated with clathrin coated vesicles. Among them are AP180 and AP-2. We and others have previously described synaptic localization of AP180. AP180 immunoreactivity is altered in both the superior frontal gyrus and hippocampus in Alzheimer's disease (AD). We here investigate the location and alteration of another adaptor protein, AP-2. In contrast to AP180, we have found that AP-2 is expressed by both neurons and glia. Furthermore, the only noticeable change of AP-2 in AD is a loss of its immunoreactivity in layer II of the superior frontal gyrus.

Original language	English (US)
Pages (from-to)	921-929
Number of pages	9
Journal	Neurobiology of Aging
Volume	21
Issue number	6
DOIs	https://doi.org/10.1016/S0197-4580(00)00228-1
State	Published - 2000
Externally published	Yes

Keywords

AP-2
Alzheimer's disease
Clathrin assembly protein
Endocytosis
Immunoblot analysis
Immunohistochemistry
Synaptic vesicle recycling

ASJC Scopus subject areas

General Neuroscience
Aging
Clinical Neurology
Developmental Biology
Geriatrics and Gerontology

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10.1016/S0197-4580(00)00228-1

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title = "Clathrin assembly protein AP-2 is detected in both neurons and glia, and its reduction is prominent in layer II of frontal cortex in Alzheimer's disease",

abstract = "There are several adaptor proteins associated with clathrin coated vesicles. Among them are AP180 and AP-2. We and others have previously described synaptic localization of AP180. AP180 immunoreactivity is altered in both the superior frontal gyrus and hippocampus in Alzheimer's disease (AD). We here investigate the location and alteration of another adaptor protein, AP-2. In contrast to AP180, we have found that AP-2 is expressed by both neurons and glia. Furthermore, the only noticeable change of AP-2 in AD is a loss of its immunoreactivity in layer II of the superior frontal gyrus.",

keywords = "AP-2, Alzheimer's disease, Clathrin assembly protein, Endocytosis, Immunoblot analysis, Immunohistochemistry, Synaptic vesicle recycling",

author = "Yao, {Pamela J.} and Weimer, {Jill M.} and O{\textquoteright}Herron, {Timothy M.} and Coleman, {Paul D.}",

note = "Funding Information: We would like to thank Royal L. Chamberlain and Vince Sullivan for their expertise and assistance in photography. We also thank Marc W. Halterman for assistance in cell line culture. This work was supported by NIH grants AG08665, AG09016, AG14441, a grant from the Lucille Markey foundation, and Pioneer Award from the Alzheimer{\textquoteright}s Association to P.D.C.",

year = "2000",

doi = "10.1016/S0197-4580(00)00228-1",

language = "English (US)",

volume = "21",

pages = "921--929",

journal = "Neurobiology of Aging",

issn = "0197-4580",

publisher = "Elsevier Inc.",

number = "6",

}

TY - JOUR

T1 - Clathrin assembly protein AP-2 is detected in both neurons and glia, and its reduction is prominent in layer II of frontal cortex in Alzheimer's disease

AU - Yao, Pamela J.

AU - Weimer, Jill M.

AU - O’Herron, Timothy M.

AU - Coleman, Paul D.

N1 - Funding Information: We would like to thank Royal L. Chamberlain and Vince Sullivan for their expertise and assistance in photography. We also thank Marc W. Halterman for assistance in cell line culture. This work was supported by NIH grants AG08665, AG09016, AG14441, a grant from the Lucille Markey foundation, and Pioneer Award from the Alzheimer’s Association to P.D.C.

PY - 2000

Y1 - 2000

N2 - There are several adaptor proteins associated with clathrin coated vesicles. Among them are AP180 and AP-2. We and others have previously described synaptic localization of AP180. AP180 immunoreactivity is altered in both the superior frontal gyrus and hippocampus in Alzheimer's disease (AD). We here investigate the location and alteration of another adaptor protein, AP-2. In contrast to AP180, we have found that AP-2 is expressed by both neurons and glia. Furthermore, the only noticeable change of AP-2 in AD is a loss of its immunoreactivity in layer II of the superior frontal gyrus.

AB - There are several adaptor proteins associated with clathrin coated vesicles. Among them are AP180 and AP-2. We and others have previously described synaptic localization of AP180. AP180 immunoreactivity is altered in both the superior frontal gyrus and hippocampus in Alzheimer's disease (AD). We here investigate the location and alteration of another adaptor protein, AP-2. In contrast to AP180, we have found that AP-2 is expressed by both neurons and glia. Furthermore, the only noticeable change of AP-2 in AD is a loss of its immunoreactivity in layer II of the superior frontal gyrus.

KW - AP-2

KW - Alzheimer's disease

KW - Clathrin assembly protein

KW - Endocytosis

KW - Immunoblot analysis

KW - Immunohistochemistry

KW - Synaptic vesicle recycling

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U2 - 10.1016/S0197-4580(00)00228-1

DO - 10.1016/S0197-4580(00)00228-1

M3 - Article

C2 - 11124443

AN - SCOPUS:0033638756

SN - 0197-4580

VL - 21

SP - 921

EP - 929

JO - Neurobiology of Aging

JF - Neurobiology of Aging

IS - 6

ER -

Clathrin assembly protein AP-2 is detected in both neurons and glia, and its reduction is prominent in layer II of frontal cortex in Alzheimer's disease

Abstract

Keywords

ASJC Scopus subject areas

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