TY - JOUR
T1 - Circulating tumor DNA analysis in patients with cancer
T2 - American society of clinical oncology and college of American pathologists joint review
AU - Merker, Jason D.
AU - Oxnard, Geoffrey R.
AU - Compton, Carolyn
AU - Diehn, Maximilian
AU - Hurley, Patricia
AU - Lazar, Alexander J.
AU - Lindeman, Neal
AU - Lockwood, Christina M.
AU - Rai, Alex J.
AU - Schilsky, Richard L.
AU - Tsimberidou, Apostolia M.
AU - Vasalos, Patricia
AU - Billman, Brooke L.
AU - Oliver, Thomas K.
AU - Bruinooge, Suanna S.
AU - Hayes, Daniel F.
AU - Turner, Nicholas C.
N1 - Funding Information:
Apostolia M. Tsimberidou Research Funding: EMD Serono (Inst), Baxter (Inst), Foundation Medicine (Inst), ONYX (Inst), Bayer AG (Inst), Boston Biomedical (Inst), Placon Therapeutics (Inst)
Publisher Copyright:
© 2018 College of American Pathologists. All rights reserved.
PY - 2018/10
Y1 - 2018/10
N2 - Purpose. - Clinical use of analytical tests to assess genomic variants in circulating tumor DNA (ctDNA) is increasing. This joint review from the American Society of Clinical Oncology and the College of American Pathologists summarizes current information about clinical ctDNA assays and provides a framework for future research. Methods. - An Expert Panel conducted a literature review on the use of ctDNA assays for solid tumors, including preanalytical variables, analytical validity, interpretation and reporting, and clinical validity and utility. Results. - The literature search identified 1338 references. Of those, 390, plus 31 references supplied by the Expert Panel, were selected for full-text review. There were 77 articles selected for inclusion. Conclusions. - The evidence indicates that testing for ctDNA is optimally performed on plasma collected in cell stabilization or EDTA tubes, with EDTA tubes processed within 6 hours of collection. Some ctDNA assays have demonstrated clinical validity and utility with certain types of advanced cancer; however, there is insufficient evidence of clinical validity and utility for the majority of ctDNA assays in advanced cancer. Evidence shows discordance between the results of ctDNA assays and genotyping tumor specimens, and supports tumor tissue genotyping to confirm undetected results from ctDNA tests. There is no evidence of clinical utility and little evidence of clinical validity of ctDNA assays in early-stage cancer, treatment monitoring, or residual disease detection. There is no evidence of clinical validity or clinical utility to suggest that ctDNA assays are useful for cancer screening, outside of a clinical trial. Given the rapid pace of research, reevaluation of the literature will shortly be required, along with the development of tools and guidance for clinical practice.
AB - Purpose. - Clinical use of analytical tests to assess genomic variants in circulating tumor DNA (ctDNA) is increasing. This joint review from the American Society of Clinical Oncology and the College of American Pathologists summarizes current information about clinical ctDNA assays and provides a framework for future research. Methods. - An Expert Panel conducted a literature review on the use of ctDNA assays for solid tumors, including preanalytical variables, analytical validity, interpretation and reporting, and clinical validity and utility. Results. - The literature search identified 1338 references. Of those, 390, plus 31 references supplied by the Expert Panel, were selected for full-text review. There were 77 articles selected for inclusion. Conclusions. - The evidence indicates that testing for ctDNA is optimally performed on plasma collected in cell stabilization or EDTA tubes, with EDTA tubes processed within 6 hours of collection. Some ctDNA assays have demonstrated clinical validity and utility with certain types of advanced cancer; however, there is insufficient evidence of clinical validity and utility for the majority of ctDNA assays in advanced cancer. Evidence shows discordance between the results of ctDNA assays and genotyping tumor specimens, and supports tumor tissue genotyping to confirm undetected results from ctDNA tests. There is no evidence of clinical utility and little evidence of clinical validity of ctDNA assays in early-stage cancer, treatment monitoring, or residual disease detection. There is no evidence of clinical validity or clinical utility to suggest that ctDNA assays are useful for cancer screening, outside of a clinical trial. Given the rapid pace of research, reevaluation of the literature will shortly be required, along with the development of tools and guidance for clinical practice.
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U2 - 10.5858/arpa.2018-0901-SA
DO - 10.5858/arpa.2018-0901-SA
M3 - Review article
C2 - 29504834
AN - SCOPUS:85050872574
SN - 0003-9985
VL - 142
SP - 1242
EP - 1253
JO - Archives of Pathology and Laboratory Medicine
JF - Archives of Pathology and Laboratory Medicine
IS - 10
ER -