Chromosomal assignment of 2900 tri- and tetranucleotide repeat markers using NIGMS somatic cell hybrid panel 2

Sara L.F. Sunden; Tom Busnga; John Beck; Ann McClain; Julie M. Gastier; Jacqueline C. Pulido; Chandri Naidu Yandava; Thomas Brody; Jelveh Ghazizadeh; James L. Weber; Geoffrey M. Duyk; Jeffrey C. Murray; Kenneth H. Buetow; V. C. Sheffield

doi:10.1006/geno.1996.0071

Chromosomal assignment of 2900 tri- and tetranucleotide repeat markers using NIGMS somatic cell hybrid panel 2

Sara L.F. Sunden, Tom Busnga, John Beck, Ann McClain, Julie M. Gastier, Jacqueline C. Pulido, Chandri Naidu Yandava, Thomas Brody, Jelveh Ghazizadeh, James L. Weber, Geoffrey M. Duyk, Jeffrey C. Murray, Kenneth H. Buetow, V. C. Sheffield

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Two thousand nine hundred and thirty-one tri- and tetranucleotide short tandem repeat polymorphisms (STRPs) developed by the Cooperative Human Linkage Center were assigned to chromosomes using the NIGMS somatic cell hybrid mapping panel 2 and an efficient pooling strategy. Approximately 82% of all STRPs tested were assigned by this method, with 96.7% accuracy. Many of the single chromosome cell lines contained portions of additional chromosomes, confirming previous reports. The cell lines for chromosomes 6, 14, and 20 contained extensive portions of other chromosomes. Five previously unreported chromosomal contaminants were identified and are reported. A new pooling strategy was designed to minimize ambiguous assignments.

Original language	English (US)
Pages (from-to)	15-20
Number of pages	6
Journal	Genomics
Volume	32
Issue number	1
DOIs	https://doi.org/10.1006/geno.1996.0071
State	Published - Feb 15 1996
Externally published	Yes

ASJC Scopus subject areas

Genetics

Access to Document

10.1006/geno.1996.0071

Cite this

Sunden, S. L. F., Busnga, T., Beck, J., McClain, A., Gastier, J. M., Pulido, J. C., Yandava, C. N., Brody, T., Ghazizadeh, J., Weber, J. L., Duyk, G. M., Murray, J. C., Buetow, K. H., & Sheffield, V. C. (1996). Chromosomal assignment of 2900 tri- and tetranucleotide repeat markers using NIGMS somatic cell hybrid panel 2. Genomics, 32(1), 15-20. https://doi.org/10.1006/geno.1996.0071

@article{256337f93943401a941d3dd04f626e1e,

title = "Chromosomal assignment of 2900 tri- and tetranucleotide repeat markers using NIGMS somatic cell hybrid panel 2",

abstract = "Two thousand nine hundred and thirty-one tri- and tetranucleotide short tandem repeat polymorphisms (STRPs) developed by the Cooperative Human Linkage Center were assigned to chromosomes using the NIGMS somatic cell hybrid mapping panel 2 and an efficient pooling strategy. Approximately 82% of all STRPs tested were assigned by this method, with 96.7% accuracy. Many of the single chromosome cell lines contained portions of additional chromosomes, confirming previous reports. The cell lines for chromosomes 6, 14, and 20 contained extensive portions of other chromosomes. Five previously unreported chromosomal contaminants were identified and are reported. A new pooling strategy was designed to minimize ambiguous assignments.",

author = "Sunden, {Sara L.F.} and Tom Busnga and John Beck and Ann McClain and Gastier, {Julie M.} and Pulido, {Jacqueline C.} and Yandava, {Chandri Naidu} and Thomas Brody and Jelveh Ghazizadeh and Weber, {James L.} and Duyk, {Geoffrey M.} and Murray, {Jeffrey C.} and Buetow, {Kenneth H.} and Sheffield, {V. C.}",

note = "Funding Information: We thank the following individuals for excellent technical assistance, without which this work would not have been possible: Gretel Beck, Dee Ann Even, Nancy Newkirk, Brian Morrison, John Poulter, Teri Randall-Hall, Tanya Rohklina, Lori Schomberg, Courtney Tchida, and Dawn Weigel. This work was supported by NIH Grants PSOHa00835 and RO1Ha0457.",

year = "1996",

month = feb,

day = "15",

doi = "10.1006/geno.1996.0071",

language = "English (US)",

volume = "32",

pages = "15--20",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Chromosomal assignment of 2900 tri- and tetranucleotide repeat markers using NIGMS somatic cell hybrid panel 2

AU - Sunden, Sara L.F.

AU - Busnga, Tom

AU - Beck, John

AU - McClain, Ann

AU - Gastier, Julie M.

AU - Pulido, Jacqueline C.

AU - Yandava, Chandri Naidu

AU - Brody, Thomas

AU - Ghazizadeh, Jelveh

AU - Weber, James L.

AU - Duyk, Geoffrey M.

AU - Murray, Jeffrey C.

AU - Buetow, Kenneth H.

AU - Sheffield, V. C.

N1 - Funding Information: We thank the following individuals for excellent technical assistance, without which this work would not have been possible: Gretel Beck, Dee Ann Even, Nancy Newkirk, Brian Morrison, John Poulter, Teri Randall-Hall, Tanya Rohklina, Lori Schomberg, Courtney Tchida, and Dawn Weigel. This work was supported by NIH Grants PSOHa00835 and RO1Ha0457.

PY - 1996/2/15

Y1 - 1996/2/15

N2 - Two thousand nine hundred and thirty-one tri- and tetranucleotide short tandem repeat polymorphisms (STRPs) developed by the Cooperative Human Linkage Center were assigned to chromosomes using the NIGMS somatic cell hybrid mapping panel 2 and an efficient pooling strategy. Approximately 82% of all STRPs tested were assigned by this method, with 96.7% accuracy. Many of the single chromosome cell lines contained portions of additional chromosomes, confirming previous reports. The cell lines for chromosomes 6, 14, and 20 contained extensive portions of other chromosomes. Five previously unreported chromosomal contaminants were identified and are reported. A new pooling strategy was designed to minimize ambiguous assignments.

AB - Two thousand nine hundred and thirty-one tri- and tetranucleotide short tandem repeat polymorphisms (STRPs) developed by the Cooperative Human Linkage Center were assigned to chromosomes using the NIGMS somatic cell hybrid mapping panel 2 and an efficient pooling strategy. Approximately 82% of all STRPs tested were assigned by this method, with 96.7% accuracy. Many of the single chromosome cell lines contained portions of additional chromosomes, confirming previous reports. The cell lines for chromosomes 6, 14, and 20 contained extensive portions of other chromosomes. Five previously unreported chromosomal contaminants were identified and are reported. A new pooling strategy was designed to minimize ambiguous assignments.

UR - http://www.scopus.com/inward/record.url?scp=17744404331&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=17744404331&partnerID=8YFLogxK

U2 - 10.1006/geno.1996.0071

DO - 10.1006/geno.1996.0071

M3 - Article

C2 - 8786107

AN - SCOPUS:17744404331

SN - 0888-7543

VL - 32

SP - 15

EP - 20

JO - Genomics

JF - Genomics

IS - 1

ER -

Chromosomal assignment of 2900 tri- and tetranucleotide repeat markers using NIGMS somatic cell hybrid panel 2

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this