Chromosomal assignment of 2900 tri- and tetranucleotide repeat markers using NIGMS somatic cell hybrid panel 2

Sara L.F. Sunden, Tom Busnga, John Beck, Ann McClain, Julie M. Gastier, Jacqueline C. Pulido, Chandri Naidu Yandava, Thomas Brody, Jelveh Ghazizadeh, James L. Weber, Geoffrey M. Duyk, Jeffrey C. Murray, Kenneth H. Buetow, V. C. Sheffield

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Two thousand nine hundred and thirty-one tri- and tetranucleotide short tandem repeat polymorphisms (STRPs) developed by the Cooperative Human Linkage Center were assigned to chromosomes using the NIGMS somatic cell hybrid mapping panel 2 and an efficient pooling strategy. Approximately 82% of all STRPs tested were assigned by this method, with 96.7% accuracy. Many of the single chromosome cell lines contained portions of additional chromosomes, confirming previous reports. The cell lines for chromosomes 6, 14, and 20 contained extensive portions of other chromosomes. Five previously unreported chromosomal contaminants were identified and are reported. A new pooling strategy was designed to minimize ambiguous assignments.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalGenomics
Volume32
Issue number1
DOIs
StatePublished - Feb 15 1996
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Sunden, S. L. F., Busnga, T., Beck, J., McClain, A., Gastier, J. M., Pulido, J. C., Yandava, C. N., Brody, T., Ghazizadeh, J., Weber, J. L., Duyk, G. M., Murray, J. C., Buetow, K. H., & Sheffield, V. C. (1996). Chromosomal assignment of 2900 tri- and tetranucleotide repeat markers using NIGMS somatic cell hybrid panel 2. Genomics, 32(1), 15-20. https://doi.org/10.1006/geno.1996.0071