Chloroplast phosphoproteins: regulation of excitation energy transfer by phosphorylation of thylakoid membrane polypeptides.

J. Bennett, K. E. Steinback, C. J. Arntzen

Research output: Contribution to journalArticle

254 Citations (Scopus)

Abstract

Incubation of isolated chloroplast thylakoid membranes with [gamma-32P]ATP results in phosphorylation of surface-exposed segments of several membrane proteins. The incorporation of 32P is light dependent, is blocked by 3(3,4-dichlorophenyl)-1,1-dimethylurea (diuron, an inhibitor of electron transport), but is insensitive to uncouplers of photophosphorylation. Polypeptides of the light-harvesting chlorophyll a/b-protein complex are the major phosphorylated membrane proteins. Addition of ATP to isolated chloroplast thylakoid membranes at 20 degrees C results in a time-dependent reduction of chlorophyll fluorescence emission; this is blocked by diuron but not by nigericin. ADP could not substitute for ATP. Chlorophyll fluorescence induction transients showed a decrease in the variable component after incubation of the membranes with ATP. Chlorophyll fluorescence at 77 K of phosphorylated thylakoid membranes showed an increase in long-wavelength emission compared with dephosphorylated controls. We conclude that a membrane-bound protein kinase can phosphorylate surface-exposed segments of the light-harvesting pigment-protein complex, altering the properties of its interaction with the two photosystems such that the distribution of absorbed excitation energy increasingly favors photosystem I.

Original languageEnglish (US)
Pages (from-to)5253-5257
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number9
StatePublished - Sep 1980
Externally publishedYes

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Thylakoids
Phosphoproteins
Energy Transfer
Chloroplasts
Diuron
Chlorophyll
Adenosine Triphosphate
Phosphorylation
Membrane Proteins
Fluorescence
Light-Harvesting Protein Complexes
Photophosphorylation
Chlorophyll Binding Proteins
Nigericin
Photosystem I Protein Complex
Light
Electron Transport
Adenosine Diphosphate
Protein Kinases
Peptides

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Chloroplast phosphoproteins : regulation of excitation energy transfer by phosphorylation of thylakoid membrane polypeptides. / Bennett, J.; Steinback, K. E.; Arntzen, C. J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 77, No. 9, 09.1980, p. 5253-5257.

Research output: Contribution to journalArticle

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