CHLOROPHYLL PHOTOSENSITIZED ELECTRON TRANSFER REACTIONS IN LIPID BILAYER VESICLES

GENERATION OF PROTON GRADIENTS ACROSS THE BILAYER COUPLED TO QUINONE REDUCTION AND HYDROQUINONE OXIDATION

Zhan-Gong Zhao, Gordon Tollin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Abstract— A chlorophyll‐containing small unilamellar lipid bilayer vesicle system with a sulfonated quinone molecule (MQS) in one aqueous compartment and a sulfonated hydroquinone molecule (H2QS) in the other has been investigated, using laser flash photolysis and steady‐state irradiation, as a means of storing light energy in the form of a proton gradient across the lipid bilayer. Under optimal conditions, an efficiency of 39% based on the chlorophyll triplet state quenched has been achieved for vectorial electron transfer across the bilayer; this corresponds to a quantum yield of 23% based on absorbed photons. As a consequence of irradiation by a single laser flash, 0.2 μM of protons were taken up by quinone reduction (MQS → H2MQS) in the outer compartment. The same number of protons were released in the inner compartment by hydroquinone oxidation (H2QS → QS). Since the volume occupied by the vesicles was only 1/1000 of the total volume of the sample, the local concentration of protons in the inner compartment was 1000 times larger (i.e.≅ 200 μM), resulting in the generation of an appreciable proton gradient across the bilayer.

Original languageEnglish (US)
Pages (from-to)611-619
Number of pages9
JournalPhotochemistry and Photobiology
Volume55
Issue number4
DOIs
StatePublished - Jan 1 1992

Fingerprint

Lipid bilayers
Lipid Bilayers
quinones
chlorophylls
Chlorophyll
lipids
Protons
compartments
electron transfer
Electrons
Oxidation
gradients
oxidation
protons
flash
Lasers
Irradiation
Molecules
irradiation
Photolysis

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

Cite this

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title = "CHLOROPHYLL PHOTOSENSITIZED ELECTRON TRANSFER REACTIONS IN LIPID BILAYER VESICLES: GENERATION OF PROTON GRADIENTS ACROSS THE BILAYER COUPLED TO QUINONE REDUCTION AND HYDROQUINONE OXIDATION",
abstract = "Abstract— A chlorophyll‐containing small unilamellar lipid bilayer vesicle system with a sulfonated quinone molecule (MQS) in one aqueous compartment and a sulfonated hydroquinone molecule (H2QS) in the other has been investigated, using laser flash photolysis and steady‐state irradiation, as a means of storing light energy in the form of a proton gradient across the lipid bilayer. Under optimal conditions, an efficiency of 39{\%} based on the chlorophyll triplet state quenched has been achieved for vectorial electron transfer across the bilayer; this corresponds to a quantum yield of 23{\%} based on absorbed photons. As a consequence of irradiation by a single laser flash, 0.2 μM of protons were taken up by quinone reduction (MQS → H2MQS) in the outer compartment. The same number of protons were released in the inner compartment by hydroquinone oxidation (H2QS → QS). Since the volume occupied by the vesicles was only 1/1000 of the total volume of the sample, the local concentration of protons in the inner compartment was 1000 times larger (i.e.≅ 200 μM), resulting in the generation of an appreciable proton gradient across the bilayer.",
author = "Zhan-Gong Zhao and Gordon Tollin",
year = "1992",
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T1 - CHLOROPHYLL PHOTOSENSITIZED ELECTRON TRANSFER REACTIONS IN LIPID BILAYER VESICLES

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AU - Zhao, Zhan-Gong

AU - Tollin, Gordon

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N2 - Abstract— A chlorophyll‐containing small unilamellar lipid bilayer vesicle system with a sulfonated quinone molecule (MQS) in one aqueous compartment and a sulfonated hydroquinone molecule (H2QS) in the other has been investigated, using laser flash photolysis and steady‐state irradiation, as a means of storing light energy in the form of a proton gradient across the lipid bilayer. Under optimal conditions, an efficiency of 39% based on the chlorophyll triplet state quenched has been achieved for vectorial electron transfer across the bilayer; this corresponds to a quantum yield of 23% based on absorbed photons. As a consequence of irradiation by a single laser flash, 0.2 μM of protons were taken up by quinone reduction (MQS → H2MQS) in the outer compartment. The same number of protons were released in the inner compartment by hydroquinone oxidation (H2QS → QS). Since the volume occupied by the vesicles was only 1/1000 of the total volume of the sample, the local concentration of protons in the inner compartment was 1000 times larger (i.e.≅ 200 μM), resulting in the generation of an appreciable proton gradient across the bilayer.

AB - Abstract— A chlorophyll‐containing small unilamellar lipid bilayer vesicle system with a sulfonated quinone molecule (MQS) in one aqueous compartment and a sulfonated hydroquinone molecule (H2QS) in the other has been investigated, using laser flash photolysis and steady‐state irradiation, as a means of storing light energy in the form of a proton gradient across the lipid bilayer. Under optimal conditions, an efficiency of 39% based on the chlorophyll triplet state quenched has been achieved for vectorial electron transfer across the bilayer; this corresponds to a quantum yield of 23% based on absorbed photons. As a consequence of irradiation by a single laser flash, 0.2 μM of protons were taken up by quinone reduction (MQS → H2MQS) in the outer compartment. The same number of protons were released in the inner compartment by hydroquinone oxidation (H2QS → QS). Since the volume occupied by the vesicles was only 1/1000 of the total volume of the sample, the local concentration of protons in the inner compartment was 1000 times larger (i.e.≅ 200 μM), resulting in the generation of an appreciable proton gradient across the bilayer.

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