Chimeric origins of ochrophytes and haptophytes revealed through an ancient plastid proteome

Richard G. Dorrell, Gillian Gile, Giselle McCallum, Raphaël Méheust, Eric P. Bapteste, Christen M. Klinger, Loraine Brillet-Guéguen, Katalina D. Freeman, Daniel J. Richter, Chris Bowler

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Plastids are supported by a wide range of proteins encoded within the nucleus and imported from the cytoplasm. These plastid-targeted proteins may originate from the endosymbiont, the host, or other sources entirely. Here, we identify and characterise 770 plastid- targeted proteins that are conserved across the ochrophytes, a major group of algae including diatoms, pelagophytes and kelps, that possess plastids derived from red algae. We show that the ancestral ochrophyte plastid proteome was an evolutionary chimera, with 25% of its phylogenetically tractable nucleus-encoded proteins deriving from green algae. We additionally show that functional mixing of host and plastid proteomes, such as through dual-targeting, is an ancestral feature of plastid evolution. Finally, we detect a clear phylogenetic signal from one ochrophyte subgroup, the lineage containing pelagophytes and dictyochophytes, in plastid- targeted proteins from another major algal lineage, the haptophytes. This may represent a possible serial endosymbiosis event deep in eukaryotic evolutionary history.

Original languageEnglish (US)
Article numbere23717
JournaleLife
Volume6
DOIs
StatePublished - May 12 2017

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Haptophyta
Plastids
Proteome
Chloroplast Proteins
Algae
Proteins
Rhodophyta
Diatoms
Chlorophyta
Symbiosis
Cytoplasm
History

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Dorrell, R. G., Gile, G., McCallum, G., Méheust, R., Bapteste, E. P., Klinger, C. M., ... Bowler, C. (2017). Chimeric origins of ochrophytes and haptophytes revealed through an ancient plastid proteome. eLife, 6, [e23717]. https://doi.org/10.7554/eLife.23717

Chimeric origins of ochrophytes and haptophytes revealed through an ancient plastid proteome. / Dorrell, Richard G.; Gile, Gillian; McCallum, Giselle; Méheust, Raphaël; Bapteste, Eric P.; Klinger, Christen M.; Brillet-Guéguen, Loraine; Freeman, Katalina D.; Richter, Daniel J.; Bowler, Chris.

In: eLife, Vol. 6, e23717, 12.05.2017.

Research output: Contribution to journalArticle

Dorrell, RG, Gile, G, McCallum, G, Méheust, R, Bapteste, EP, Klinger, CM, Brillet-Guéguen, L, Freeman, KD, Richter, DJ & Bowler, C 2017, 'Chimeric origins of ochrophytes and haptophytes revealed through an ancient plastid proteome', eLife, vol. 6, e23717. https://doi.org/10.7554/eLife.23717
Dorrell RG, Gile G, McCallum G, Méheust R, Bapteste EP, Klinger CM et al. Chimeric origins of ochrophytes and haptophytes revealed through an ancient plastid proteome. eLife. 2017 May 12;6. e23717. https://doi.org/10.7554/eLife.23717
Dorrell, Richard G. ; Gile, Gillian ; McCallum, Giselle ; Méheust, Raphaël ; Bapteste, Eric P. ; Klinger, Christen M. ; Brillet-Guéguen, Loraine ; Freeman, Katalina D. ; Richter, Daniel J. ; Bowler, Chris. / Chimeric origins of ochrophytes and haptophytes revealed through an ancient plastid proteome. In: eLife. 2017 ; Vol. 6.
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