Chemomechanical coupling in hexameric protein-protein interfaces harnesses energy within V-type ATPases

Abhishek Singharoy, Christophe Chipot, Mahmoud Moradi, Klaus Schulten

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

ATP synthase is the most prominent bioenergetic macromolecular motor in all life forms, utilizing the proton gradient across the cell membrane to fuel the synthesis of ATP. Notwithstanding the wealth of available biochemical and structural information inferred from years of experiments, the precise molecular mechanism whereby vacuolar (V-type) ATP synthase fulfills its biological function remains largely fragmentary. Recently, crystallographers provided the first high-resolution view of ATP activity in Enterococcus hirae V1-ATPase. Employing a combination of transition-path sampling and high-performance free-energy methods, the sequence of conformational transitions involved in a functional cycle accompanying ATP hydrolysis has been investigated in unprecedented detail over an aggregate simulation time of 65 μs. Our simulated pathways reveal that the chemical energy produced by ATP hydrolysis is harnessed via the concerted motion of the protein-protein interfaces in the V1-ring, and is nearly entirely consumed in the rotation of the central stalk. Surprisingly, in an ATPase devoid of a central stalk, the interfaces of this ring are perfectly designed for inducing ATP hydrolysis. However, in a complete V1-ATPase, the mechanical property of the central stalk is a key determinant of the rate of ATP turnover. The simulations further unveil a sequence of events, whereby unbinding of the hydrolysis product (ADP + Pi) is followed by ATP uptake, which, in turn, leads to the torque generation step and rotation of the center stalk. Molecular trajectories also bring to light multiple intermediates, two of which have been isolated in independent crystallography experiments.

Original languageEnglish (US)
Pages (from-to)293-310
Number of pages18
JournalJournal of the American Chemical Society
Volume139
Issue number1
DOIs
StatePublished - Jan 11 2017
Externally publishedYes

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Vacuolar Proton-Translocating ATPases
Adenosinetriphosphate
Adenosine Triphosphate
Proteins
Hydrolysis
Adenosine Triphosphatases
Administrative data processing
Crystallography
Torque
Cell membranes
Adenosine Diphosphate
Energy Metabolism
Free energy
Protons
Experiments
Trajectories
Cell Membrane
Sampling
Mechanical properties

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Chemomechanical coupling in hexameric protein-protein interfaces harnesses energy within V-type ATPases. / Singharoy, Abhishek; Chipot, Christophe; Moradi, Mahmoud; Schulten, Klaus.

In: Journal of the American Chemical Society, Vol. 139, No. 1, 11.01.2017, p. 293-310.

Research output: Contribution to journalArticle

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