Chemical recognition and binding kinetics in a functionalized tunnel junction

Shuai Chang, Shuo Huang, Hao Liu, Peiming Zhang, Feng Liang, Rena Akahori, Shengqin Li, Brett Gyarfas, John Shumway, Brian Ashcroft, Jin He, Stuart Lindsay

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

4(5)-(2-mercaptoethyl)-1H-imidazole-2-carboxamide is a molecule that has multiple hydrogen bonding sites and a short flexible linker. When tethered to a pair of electrodes, it traps target molecules in a tunnel junction. Surprisingly large recognition-tunneling signals are generated for all naturally occurring DNA bases A, C, G, T and 5-methyl-cytosine. Tunnel current spikes are stochastic and broadly distributed, but characteristic enough so that individual bases can be identified as a tunneling probe is scanned over DNA oligomers. Each base yields a recognizable burst of signal, the duration of which is controlled entirely by the probe speed, down to speeds of 1nms 1, implying a maximum off-rate of 3s 1 for the recognition complex. The same measurements yield a lower bound on the on-rate of 1M 1s 1. Despite the stochastic nature of the signals, an optimized multiparameter fit allows base calling from a single signal peak with an accuracy that can exceed 80% when a single type of nucleotide is present in the junction, meaning that recognition-tunneling is capable of true single-molecule analysis. The accuracy increases to 95% when multiple spikes in a signal cluster are analyzed.

Original languageEnglish (US)
Article number235101
JournalNanotechnology
Volume23
Issue number23
DOIs
StatePublished - Jun 15 2012

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Tunnel junctions
Molecules
Kinetics
DNA
Cytosine
Nucleotides
Oligomers
Tunnels
Hydrogen bonds
Electrodes

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

Chemical recognition and binding kinetics in a functionalized tunnel junction. / Chang, Shuai; Huang, Shuo; Liu, Hao; Zhang, Peiming; Liang, Feng; Akahori, Rena; Li, Shengqin; Gyarfas, Brett; Shumway, John; Ashcroft, Brian; He, Jin; Lindsay, Stuart.

In: Nanotechnology, Vol. 23, No. 23, 235101, 15.06.2012.

Research output: Contribution to journalArticle

Chang, S, Huang, S, Liu, H, Zhang, P, Liang, F, Akahori, R, Li, S, Gyarfas, B, Shumway, J, Ashcroft, B, He, J & Lindsay, S 2012, 'Chemical recognition and binding kinetics in a functionalized tunnel junction', Nanotechnology, vol. 23, no. 23, 235101. https://doi.org/10.1088/0957-4484/23/23/235101
Chang S, Huang S, Liu H, Zhang P, Liang F, Akahori R et al. Chemical recognition and binding kinetics in a functionalized tunnel junction. Nanotechnology. 2012 Jun 15;23(23). 235101. https://doi.org/10.1088/0957-4484/23/23/235101
Chang, Shuai ; Huang, Shuo ; Liu, Hao ; Zhang, Peiming ; Liang, Feng ; Akahori, Rena ; Li, Shengqin ; Gyarfas, Brett ; Shumway, John ; Ashcroft, Brian ; He, Jin ; Lindsay, Stuart. / Chemical recognition and binding kinetics in a functionalized tunnel junction. In: Nanotechnology. 2012 ; Vol. 23, No. 23.
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