The preparation of transfer RNA's terminating in 2′- and 3′-O-methyl-adenosine has been undertaken by a combination of chemical and enzymatic techniques to permit a determination of the mechanism of tRNA aminoacylation. Neither of the two synthetic tRNA's was aminoacylated substantially in the presence of aminoacyl-tRNA synthetases, consistent with the hypothesis that the aminoacyladenylate moiety exists in the form of a species with considerable orthoester character.
|Original language||English (US)|
|Number of pages||7|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Jun 19 1973|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology