Chemical modifications of transfer RNA species. Transfer RNA's terminating in 2′- and 3′-O-methyladenosine

Sidney Hecht, S. D. Hawrelak, John W. Kozarich, Francis J. Schmidt, Robert M. Bock

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The preparation of transfer RNA's terminating in 2′- and 3′-O-methyl-adenosine has been undertaken by a combination of chemical and enzymatic techniques to permit a determination of the mechanism of tRNA aminoacylation. Neither of the two synthetic tRNA's was aminoacylated substantially in the presence of aminoacyl-tRNA synthetases, consistent with the hypothesis that the aminoacyladenylate moiety exists in the form of a species with considerable orthoester character.

Original languageEnglish (US)
Pages (from-to)1341-1347
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume52
Issue number4
DOIs
StatePublished - Jun 19 1973
Externally publishedYes

Fingerprint

Chemical modification
Transfer RNA
Transfer RNA Aminoacylation
Amino Acyl-tRNA Synthetases
Adenosine
3'-O-methyladenosine
2'-O-methyladenosine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Chemical modifications of transfer RNA species. Transfer RNA's terminating in 2′- and 3′-O-methyladenosine. / Hecht, Sidney; Hawrelak, S. D.; Kozarich, John W.; Schmidt, Francis J.; Bock, Robert M.

In: Biochemical and Biophysical Research Communications, Vol. 52, No. 4, 19.06.1973, p. 1341-1347.

Research output: Contribution to journalArticle

Hecht, Sidney ; Hawrelak, S. D. ; Kozarich, John W. ; Schmidt, Francis J. ; Bock, Robert M. / Chemical modifications of transfer RNA species. Transfer RNA's terminating in 2′- and 3′-O-methyladenosine. In: Biochemical and Biophysical Research Communications. 1973 ; Vol. 52, No. 4. pp. 1341-1347.
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