Chemical modifications of transfer RNA species. Transfer RNA's terminating in 2′- and 3′-O-methyladenosine

Sidney M. Hecht; S. D. Hawrelak; John W. Kozarich; Francis J. Schmidt; Robert M. Bock

doi:10.1016/0006-291X(73)90648-7

Chemical modifications of transfer RNA species. Transfer RNA's terminating in 2′- and 3′-O-methyladenosine

Sidney M. Hecht, S. D. Hawrelak, John W. Kozarich, Francis J. Schmidt, Robert M. Bock

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The preparation of transfer RNA's terminating in 2′- and 3′-O-methyl-adenosine has been undertaken by a combination of chemical and enzymatic techniques to permit a determination of the mechanism of tRNA aminoacylation. Neither of the two synthetic tRNA's was aminoacylated substantially in the presence of aminoacyl-tRNA synthetases, consistent with the hypothesis that the aminoacyladenylate moiety exists in the form of a species with considerable orthoester character.

Original language	English (US)
Pages (from-to)	1341-1347
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	52
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(73)90648-7
State	Published - Jun 19 1973
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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AU - Hecht, Sidney M.

AU - Hawrelak, S. D.

AU - Kozarich, John W.

AU - Schmidt, Francis J.

AU - Bock, Robert M.

PY - 1973/6/19

Y1 - 1973/6/19

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AB - The preparation of transfer RNA's terminating in 2′- and 3′-O-methyl-adenosine has been undertaken by a combination of chemical and enzymatic techniques to permit a determination of the mechanism of tRNA aminoacylation. Neither of the two synthetic tRNA's was aminoacylated substantially in the presence of aminoacyl-tRNA synthetases, consistent with the hypothesis that the aminoacyladenylate moiety exists in the form of a species with considerable orthoester character.

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Chemical modifications of transfer RNA species. Transfer RNA's terminating in 2′- and 3′-O-methyladenosine

Abstract

ASJC Scopus subject areas

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