Chemical modifications of transfer RNA species. Transfer RNA's terminating in 2′- and 3′-O-methyladenosine

Sidney M. Hecht, S. D. Hawrelak, John W. Kozarich, Francis J. Schmidt, Robert M. Bock

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The preparation of transfer RNA's terminating in 2′- and 3′-O-methyl-adenosine has been undertaken by a combination of chemical and enzymatic techniques to permit a determination of the mechanism of tRNA aminoacylation. Neither of the two synthetic tRNA's was aminoacylated substantially in the presence of aminoacyl-tRNA synthetases, consistent with the hypothesis that the aminoacyladenylate moiety exists in the form of a species with considerable orthoester character.

Original languageEnglish (US)
Pages (from-to)1341-1347
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume52
Issue number4
DOIs
StatePublished - Jun 19 1973
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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