Charge-Sensitive Optical Detection of Binding Kinetics between Phage-Displayed Peptide Ligands and Protein Targets

Runli Liang, Yingnan Zhang, Guangzhong Ma, Shaopeng Wang

Research output: Contribution to journalArticlepeer-review

Abstract

Phage display technology has been a powerful tool in peptide drug development. However, the supremacy of phage display-based peptide drug discovery is plagued by the follow-up process of peptides synthesis, which is costly and time consuming, but is necessary for the accurate measurement of binding kinetics in order to properly triage the best peptide leads during the affinity maturation stages. A sensitive technology is needed for directly measuring the binding kinetics of peptides on phages to reduce the time and cost of the entire process. Here, we show the capability of a charge-sensitive optical detection (CSOD) method for the direct quantification of binding kinetics of phage-displayed peptides to their target protein, using whole phages. We anticipate CSOD will contribute to streamline the process of phage display-based drug discovery.

Original languageEnglish (US)
Article number394
JournalBiosensors
Volume12
Issue number6
DOIs
StatePublished - Jun 2022

Keywords

  • binding kinetics
  • charge-sensitive optical detection
  • label-free
  • peptide
  • phage-display
  • protein

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biotechnology
  • Biomedical Engineering
  • Instrumentation
  • Engineering (miscellaneous)
  • Clinical Biochemistry

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