Characterizing the secondary protein structure of black widow dragline silk using solid-state NMR and X-ray diffraction

Janelle E. Jenkins, Sujatha Sampath, Emily Butler, Jihyun Kim, Robert W. Henning, Gregory P. Holland, Jeffery Yarger

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

This study provides a detailed secondary structural characterization of major ampullate dragline silk from Latrodectus hesperus (black widow) spiders. X-ray diffraction results show that the structure of black widow major ampullate silk fibers is comprised of stacked β-sheet nanocrystallites oriented parallel to the fiber axis and an amorphous region with oriented (anisotropic) and isotropic components. The combination of two-dimensional (2D) 13C-13C through-space and through-bond solid-state NMR experiments provide chemical shifts that are used to determine detailed information about the amino acid motif secondary structure in black widow spider dragline silk. Individual amino acids are incorporated into different repetitive motifs that make up the majority of this protein-based biopolymer. From the solid-state NMR measurements, we assign distinct secondary conformations to each repetitive amino acid motif and, hence, to the amino acids that make up the motifs. Specifically, alanine is incorporated in β-sheet (poly(Alan) and poly(Gly-Ala)), 31-helix (poly(Gly-Gly-Xaa), and α-helix (poly(Gln-Gln-Ala-Tyr)) components. Glycine is determined to be in β-sheet (poly(Gly-Ala)) and 31-helical (poly(Gly-Gly-Xaa)) regions, while serine is present in β-sheet (poly(Gly-Ala-Ser)), 31-helix (poly(Gly-Gly-Ser)), and β-turn (poly(Gly-Pro-Ser)) structures. These various motif-specific secondary structural elements are quantitatively correlated to the primary amino acid sequence of major ampullate spidroin 1 and 2 (MaSp1 and MaSp2) and are shown to form a self-consistent model for black widow dragline silk.

Original languageEnglish (US)
Pages (from-to)3472-3483
Number of pages12
JournalBiomacromolecules
Volume14
Issue number10
DOIs
StatePublished - Oct 14 2013

Fingerprint

Secondary Protein Structure
Silk
X-Ray Diffraction
Amino acids
Black Widow Spider
Nuclear magnetic resonance
Proteins
Amino Acids
X ray diffraction
Amino Acid Motifs
Biopolymers
Nanocrystallites
Alanine
Glycine
Serine
Fibers
Chemical shift
Amino Acid Sequence
Conformations
poly(alanylglycine)

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials
  • Medicine(all)

Cite this

Characterizing the secondary protein structure of black widow dragline silk using solid-state NMR and X-ray diffraction. / Jenkins, Janelle E.; Sampath, Sujatha; Butler, Emily; Kim, Jihyun; Henning, Robert W.; Holland, Gregory P.; Yarger, Jeffery.

In: Biomacromolecules, Vol. 14, No. 10, 14.10.2013, p. 3472-3483.

Research output: Contribution to journalArticle

Jenkins, Janelle E. ; Sampath, Sujatha ; Butler, Emily ; Kim, Jihyun ; Henning, Robert W. ; Holland, Gregory P. ; Yarger, Jeffery. / Characterizing the secondary protein structure of black widow dragline silk using solid-state NMR and X-ray diffraction. In: Biomacromolecules. 2013 ; Vol. 14, No. 10. pp. 3472-3483.
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