Abstract

Fluorescence- based lateral flow immunoassays (LFIAs) remain relatively unexplored compared to colorimetric LFIAs for point-of-care (PoC) disease diagnosis and health monitoring. For fluorescence-based LFIAs, a major challenge includes the auto-fluorescence of the nitrocellulose and nonspecific binding of fluorescent polystyrene microspheres. In this paper, we aim to characterize antibody-microsphere conjugates in a fluorescence-based serological assay on nitrocellulose. Factors such as coating concentration and quantity of microspheres were considered and their impacts on nonspecific binding and signal-to-noise ratio are discussed. Finally, we use the determined conditions for the antibody-microsphere conjugates to demonstrate the sensitivity of a proof-of-concept assay detecting antibodies to Epstein-Barr Nuclear Antigen-1 in pooled human plasma samples. A titration of the seropositive plasma samples demonstrated a titer approaching 1:1,000 using only 30 μL of diluted sample and a sample-to-result time of less than one hour.

Original languageEnglish (US)
Title of host publication2018 IEEE Life Sciences Conference, LSC 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages199-202
Number of pages4
ISBN (Electronic)9781538667095
DOIs
StatePublished - Dec 10 2018
Event2018 IEEE Life Sciences Conference, LSC 2018 - Montreal, Canada
Duration: Oct 28 2018Oct 30 2018

Other

Other2018 IEEE Life Sciences Conference, LSC 2018
CountryCanada
CityMontreal
Period10/28/1810/30/18

Fingerprint

immunoassay
antibodies
Microspheres
Immunoassay
Antibodies
Fluorescence
cellulose nitrate
Nitrocellulose
fluorescence
Collodion
Assays
Point-of-Care Systems
Plasma (human)
Nuclear Antigens
Polystyrenes
Signal-To-Noise Ratio
antigens
Antigens
Titration
titration

ASJC Scopus subject areas

  • Signal Processing
  • Medicine (miscellaneous)
  • Health Informatics
  • Instrumentation

Cite this

Zhu, M., Hou, C. W., Obahiagbon, U., Anderson, K., & Blain Christen, J. (2018). Characterizing antibody-microsphere conjugates for fluorescence-based lateral flow immunoassays. In 2018 IEEE Life Sciences Conference, LSC 2018 (pp. 199-202). [8572152] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/LSC.2018.8572152

Characterizing antibody-microsphere conjugates for fluorescence-based lateral flow immunoassays. / Zhu, Meilin; Hou, Ching Wen; Obahiagbon, Uwadiae; Anderson, Karen; Blain Christen, Jennifer.

2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. p. 199-202 8572152.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Zhu, M, Hou, CW, Obahiagbon, U, Anderson, K & Blain Christen, J 2018, Characterizing antibody-microsphere conjugates for fluorescence-based lateral flow immunoassays. in 2018 IEEE Life Sciences Conference, LSC 2018., 8572152, Institute of Electrical and Electronics Engineers Inc., pp. 199-202, 2018 IEEE Life Sciences Conference, LSC 2018, Montreal, Canada, 10/28/18. https://doi.org/10.1109/LSC.2018.8572152
Zhu M, Hou CW, Obahiagbon U, Anderson K, Blain Christen J. Characterizing antibody-microsphere conjugates for fluorescence-based lateral flow immunoassays. In 2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc. 2018. p. 199-202. 8572152 https://doi.org/10.1109/LSC.2018.8572152
Zhu, Meilin ; Hou, Ching Wen ; Obahiagbon, Uwadiae ; Anderson, Karen ; Blain Christen, Jennifer. / Characterizing antibody-microsphere conjugates for fluorescence-based lateral flow immunoassays. 2018 IEEE Life Sciences Conference, LSC 2018. Institute of Electrical and Electronics Engineers Inc., 2018. pp. 199-202
@inproceedings{6df28638b6af468bac23e3b71b845839,
title = "Characterizing antibody-microsphere conjugates for fluorescence-based lateral flow immunoassays",
abstract = "Fluorescence- based lateral flow immunoassays (LFIAs) remain relatively unexplored compared to colorimetric LFIAs for point-of-care (PoC) disease diagnosis and health monitoring. For fluorescence-based LFIAs, a major challenge includes the auto-fluorescence of the nitrocellulose and nonspecific binding of fluorescent polystyrene microspheres. In this paper, we aim to characterize antibody-microsphere conjugates in a fluorescence-based serological assay on nitrocellulose. Factors such as coating concentration and quantity of microspheres were considered and their impacts on nonspecific binding and signal-to-noise ratio are discussed. Finally, we use the determined conditions for the antibody-microsphere conjugates to demonstrate the sensitivity of a proof-of-concept assay detecting antibodies to Epstein-Barr Nuclear Antigen-1 in pooled human plasma samples. A titration of the seropositive plasma samples demonstrated a titer approaching 1:1,000 using only 30 μL of diluted sample and a sample-to-result time of less than one hour.",
author = "Meilin Zhu and Hou, {Ching Wen} and Uwadiae Obahiagbon and Karen Anderson and {Blain Christen}, Jennifer",
year = "2018",
month = "12",
day = "10",
doi = "10.1109/LSC.2018.8572152",
language = "English (US)",
pages = "199--202",
booktitle = "2018 IEEE Life Sciences Conference, LSC 2018",
publisher = "Institute of Electrical and Electronics Engineers Inc.",

}

TY - GEN

T1 - Characterizing antibody-microsphere conjugates for fluorescence-based lateral flow immunoassays

AU - Zhu, Meilin

AU - Hou, Ching Wen

AU - Obahiagbon, Uwadiae

AU - Anderson, Karen

AU - Blain Christen, Jennifer

PY - 2018/12/10

Y1 - 2018/12/10

N2 - Fluorescence- based lateral flow immunoassays (LFIAs) remain relatively unexplored compared to colorimetric LFIAs for point-of-care (PoC) disease diagnosis and health monitoring. For fluorescence-based LFIAs, a major challenge includes the auto-fluorescence of the nitrocellulose and nonspecific binding of fluorescent polystyrene microspheres. In this paper, we aim to characterize antibody-microsphere conjugates in a fluorescence-based serological assay on nitrocellulose. Factors such as coating concentration and quantity of microspheres were considered and their impacts on nonspecific binding and signal-to-noise ratio are discussed. Finally, we use the determined conditions for the antibody-microsphere conjugates to demonstrate the sensitivity of a proof-of-concept assay detecting antibodies to Epstein-Barr Nuclear Antigen-1 in pooled human plasma samples. A titration of the seropositive plasma samples demonstrated a titer approaching 1:1,000 using only 30 μL of diluted sample and a sample-to-result time of less than one hour.

AB - Fluorescence- based lateral flow immunoassays (LFIAs) remain relatively unexplored compared to colorimetric LFIAs for point-of-care (PoC) disease diagnosis and health monitoring. For fluorescence-based LFIAs, a major challenge includes the auto-fluorescence of the nitrocellulose and nonspecific binding of fluorescent polystyrene microspheres. In this paper, we aim to characterize antibody-microsphere conjugates in a fluorescence-based serological assay on nitrocellulose. Factors such as coating concentration and quantity of microspheres were considered and their impacts on nonspecific binding and signal-to-noise ratio are discussed. Finally, we use the determined conditions for the antibody-microsphere conjugates to demonstrate the sensitivity of a proof-of-concept assay detecting antibodies to Epstein-Barr Nuclear Antigen-1 in pooled human plasma samples. A titration of the seropositive plasma samples demonstrated a titer approaching 1:1,000 using only 30 μL of diluted sample and a sample-to-result time of less than one hour.

UR - http://www.scopus.com/inward/record.url?scp=85060223056&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060223056&partnerID=8YFLogxK

U2 - 10.1109/LSC.2018.8572152

DO - 10.1109/LSC.2018.8572152

M3 - Conference contribution

SP - 199

EP - 202

BT - 2018 IEEE Life Sciences Conference, LSC 2018

PB - Institute of Electrical and Electronics Engineers Inc.

ER -