Characterizing antibody-microsphere conjugates for fluorescence-based lateral flow immunoassays

Meilin Zhu, Ching Wen Hou, Uwadiae Obahiagbon, Karen Anderson, Jennifer Blain Christen

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Fluorescence- based lateral flow immunoassays (LFIAs) remain relatively unexplored compared to colorimetric LFIAs for point-of-care (PoC) disease diagnosis and health monitoring. For fluorescence-based LFIAs, a major challenge includes the auto-fluorescence of the nitrocellulose and nonspecific binding of fluorescent polystyrene microspheres. In this paper, we aim to characterize antibody-microsphere conjugates in a fluorescence-based serological assay on nitrocellulose. Factors such as coating concentration and quantity of microspheres were considered and their impacts on nonspecific binding and signal-to-noise ratio are discussed. Finally, we use the determined conditions for the antibody-microsphere conjugates to demonstrate the sensitivity of a proof-of-concept assay detecting antibodies to Epstein-Barr Nuclear Antigen-1 in pooled human plasma samples. A titration of the seropositive plasma samples demonstrated a titer approaching 1:1,000 using only 30 μL of diluted sample and a sample-to-result time of less than one hour.

Original languageEnglish (US)
Title of host publication2018 IEEE Life Sciences Conference, LSC 2018
PublisherInstitute of Electrical and Electronics Engineers Inc.
Number of pages4
ISBN (Electronic)9781538667095
StatePublished - Dec 10 2018
Event2018 IEEE Life Sciences Conference, LSC 2018 - Montreal, Canada
Duration: Oct 28 2018Oct 30 2018


Other2018 IEEE Life Sciences Conference, LSC 2018

ASJC Scopus subject areas

  • Signal Processing
  • Medicine (miscellaneous)
  • Health Informatics
  • Instrumentation

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