TY - GEN
T1 - Characterizing antibody-microsphere conjugates for fluorescence-based lateral flow immunoassays
AU - Zhu, Meilin
AU - Hou, Ching Wen
AU - Obahiagbon, Uwadiae
AU - Anderson, Karen
AU - Blain Christen, Jennifer
N1 - Funding Information:
ACKNOWLEDGMENTS This work was supported by the National Science Foundation Smart and Connected Health [grant number IIS-1521904] SCH:INT:‘Disposable high sensitivity point of care immunosensor for multiple disease and pathogen detection and the National Cancer Institute Cancer Detection, Diagnosis, and Treatment Technologies for Global Health [grant number CA211415] Rapid Point-of-Care Detection of HPV-associated Malignancies.
Publisher Copyright:
© 2018 IEEE.
PY - 2018/12/10
Y1 - 2018/12/10
N2 - Fluorescence- based lateral flow immunoassays (LFIAs) remain relatively unexplored compared to colorimetric LFIAs for point-of-care (PoC) disease diagnosis and health monitoring. For fluorescence-based LFIAs, a major challenge includes the auto-fluorescence of the nitrocellulose and nonspecific binding of fluorescent polystyrene microspheres. In this paper, we aim to characterize antibody-microsphere conjugates in a fluorescence-based serological assay on nitrocellulose. Factors such as coating concentration and quantity of microspheres were considered and their impacts on nonspecific binding and signal-to-noise ratio are discussed. Finally, we use the determined conditions for the antibody-microsphere conjugates to demonstrate the sensitivity of a proof-of-concept assay detecting antibodies to Epstein-Barr Nuclear Antigen-1 in pooled human plasma samples. A titration of the seropositive plasma samples demonstrated a titer approaching 1:1,000 using only 30 μL of diluted sample and a sample-to-result time of less than one hour.
AB - Fluorescence- based lateral flow immunoassays (LFIAs) remain relatively unexplored compared to colorimetric LFIAs for point-of-care (PoC) disease diagnosis and health monitoring. For fluorescence-based LFIAs, a major challenge includes the auto-fluorescence of the nitrocellulose and nonspecific binding of fluorescent polystyrene microspheres. In this paper, we aim to characterize antibody-microsphere conjugates in a fluorescence-based serological assay on nitrocellulose. Factors such as coating concentration and quantity of microspheres were considered and their impacts on nonspecific binding and signal-to-noise ratio are discussed. Finally, we use the determined conditions for the antibody-microsphere conjugates to demonstrate the sensitivity of a proof-of-concept assay detecting antibodies to Epstein-Barr Nuclear Antigen-1 in pooled human plasma samples. A titration of the seropositive plasma samples demonstrated a titer approaching 1:1,000 using only 30 μL of diluted sample and a sample-to-result time of less than one hour.
UR - http://www.scopus.com/inward/record.url?scp=85060223056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85060223056&partnerID=8YFLogxK
U2 - 10.1109/LSC.2018.8572152
DO - 10.1109/LSC.2018.8572152
M3 - Conference contribution
AN - SCOPUS:85060223056
T3 - 2018 IEEE Life Sciences Conference, LSC 2018
SP - 199
EP - 202
BT - 2018 IEEE Life Sciences Conference, LSC 2018
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2018 IEEE Life Sciences Conference, LSC 2018
Y2 - 28 October 2018 through 30 October 2018
ER -