Characterization of the gene encoding the envelope fusion glycoprotein GP64 from Bombyx mori nucleopolyhedrovirus

Masmudur Rahman, Karumathil P. Gopinathan

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We describe here the characterization of the gene gp64 encoding the envelope fusion protein GP64 (open reading frame) ORF 105 from Bombyx mori nucleopolyhedrovirus (BmNPV). gp64 was transcribed from the early to late stages of infection and the transcripts were seen from 6 to 72 h post infection (hpi). The early transcripts initiated from a consensus CAGT motif while the late transcripts arose from three conserved TAAG motifs, all of which were located in the near upstream region of the coding sequence. Both early and late transcripts terminated at a run of T residues following the second polyadenylation signal located 31 nt downstream of the translation termination codon. BmGP64 protein was detectable from 6 hpi and was present in larger quantities throughout the infection process from 12 hpi, in BmNPV-infected BmN cells. The persistent presence of GP64 in BmN cells differed from the protein expression pattern of GP64 in Autographa californica multinucleocapsid nucleopolyhedrovirus infection, where the protein levels decreased significantly by late times (48 hpi). BmGP64 was located in the membrane and cytoplasm of the infected host cells and as a component of the budded virions. The production of infectious budded virus and the fusion activity were reduced when glycosylation of GP64 was inhibited.

Original languageEnglish (US)
Pages (from-to)45-57
Number of pages13
JournalVirus Research
Volume94
Issue number1
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

Fingerprint

Nucleopolyhedrovirus
Bombyx
Glycoproteins
Infection
Genes
Open Reading Frames
Proteins
Polyadenylation
Terminator Codon
Glycosylation
Virion
Cytoplasm
Viruses
Membranes

Keywords

  • Baculoviruses
  • BmNPV
  • Cell fusion
  • Gene expression profiling
  • Silkworms
  • Transcriptional regulation

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

Characterization of the gene encoding the envelope fusion glycoprotein GP64 from Bombyx mori nucleopolyhedrovirus. / Rahman, Masmudur; Gopinathan, Karumathil P.

In: Virus Research, Vol. 94, No. 1, 01.07.2003, p. 45-57.

Research output: Contribution to journalArticle

@article{14cd8bd7c1aa4f2fa77eb0931d3eabe0,
title = "Characterization of the gene encoding the envelope fusion glycoprotein GP64 from Bombyx mori nucleopolyhedrovirus",
abstract = "We describe here the characterization of the gene gp64 encoding the envelope fusion protein GP64 (open reading frame) ORF 105 from Bombyx mori nucleopolyhedrovirus (BmNPV). gp64 was transcribed from the early to late stages of infection and the transcripts were seen from 6 to 72 h post infection (hpi). The early transcripts initiated from a consensus CAGT motif while the late transcripts arose from three conserved TAAG motifs, all of which were located in the near upstream region of the coding sequence. Both early and late transcripts terminated at a run of T residues following the second polyadenylation signal located 31 nt downstream of the translation termination codon. BmGP64 protein was detectable from 6 hpi and was present in larger quantities throughout the infection process from 12 hpi, in BmNPV-infected BmN cells. The persistent presence of GP64 in BmN cells differed from the protein expression pattern of GP64 in Autographa californica multinucleocapsid nucleopolyhedrovirus infection, where the protein levels decreased significantly by late times (48 hpi). BmGP64 was located in the membrane and cytoplasm of the infected host cells and as a component of the budded virions. The production of infectious budded virus and the fusion activity were reduced when glycosylation of GP64 was inhibited.",
keywords = "Baculoviruses, BmNPV, Cell fusion, Gene expression profiling, Silkworms, Transcriptional regulation",
author = "Masmudur Rahman and Gopinathan, {Karumathil P.}",
year = "2003",
month = "7",
day = "1",
doi = "10.1016/S0168-1702(03)00123-0",
language = "English (US)",
volume = "94",
pages = "45--57",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Characterization of the gene encoding the envelope fusion glycoprotein GP64 from Bombyx mori nucleopolyhedrovirus

AU - Rahman, Masmudur

AU - Gopinathan, Karumathil P.

PY - 2003/7/1

Y1 - 2003/7/1

N2 - We describe here the characterization of the gene gp64 encoding the envelope fusion protein GP64 (open reading frame) ORF 105 from Bombyx mori nucleopolyhedrovirus (BmNPV). gp64 was transcribed from the early to late stages of infection and the transcripts were seen from 6 to 72 h post infection (hpi). The early transcripts initiated from a consensus CAGT motif while the late transcripts arose from three conserved TAAG motifs, all of which were located in the near upstream region of the coding sequence. Both early and late transcripts terminated at a run of T residues following the second polyadenylation signal located 31 nt downstream of the translation termination codon. BmGP64 protein was detectable from 6 hpi and was present in larger quantities throughout the infection process from 12 hpi, in BmNPV-infected BmN cells. The persistent presence of GP64 in BmN cells differed from the protein expression pattern of GP64 in Autographa californica multinucleocapsid nucleopolyhedrovirus infection, where the protein levels decreased significantly by late times (48 hpi). BmGP64 was located in the membrane and cytoplasm of the infected host cells and as a component of the budded virions. The production of infectious budded virus and the fusion activity were reduced when glycosylation of GP64 was inhibited.

AB - We describe here the characterization of the gene gp64 encoding the envelope fusion protein GP64 (open reading frame) ORF 105 from Bombyx mori nucleopolyhedrovirus (BmNPV). gp64 was transcribed from the early to late stages of infection and the transcripts were seen from 6 to 72 h post infection (hpi). The early transcripts initiated from a consensus CAGT motif while the late transcripts arose from three conserved TAAG motifs, all of which were located in the near upstream region of the coding sequence. Both early and late transcripts terminated at a run of T residues following the second polyadenylation signal located 31 nt downstream of the translation termination codon. BmGP64 protein was detectable from 6 hpi and was present in larger quantities throughout the infection process from 12 hpi, in BmNPV-infected BmN cells. The persistent presence of GP64 in BmN cells differed from the protein expression pattern of GP64 in Autographa californica multinucleocapsid nucleopolyhedrovirus infection, where the protein levels decreased significantly by late times (48 hpi). BmGP64 was located in the membrane and cytoplasm of the infected host cells and as a component of the budded virions. The production of infectious budded virus and the fusion activity were reduced when glycosylation of GP64 was inhibited.

KW - Baculoviruses

KW - BmNPV

KW - Cell fusion

KW - Gene expression profiling

KW - Silkworms

KW - Transcriptional regulation

UR - http://www.scopus.com/inward/record.url?scp=0038433230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038433230&partnerID=8YFLogxK

U2 - 10.1016/S0168-1702(03)00123-0

DO - 10.1016/S0168-1702(03)00123-0

M3 - Article

VL - 94

SP - 45

EP - 57

JO - Virus Research

JF - Virus Research

SN - 0168-1702

IS - 1

ER -