Characterization of the gene encoding the envelope fusion glycoprotein GP64 from Bombyx mori nucleopolyhedrovirus

Md Masmudur Rahman, Karumathil P. Gopinathan

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

We describe here the characterization of the gene gp64 encoding the envelope fusion protein GP64 (open reading frame) ORF 105 from Bombyx mori nucleopolyhedrovirus (BmNPV). gp64 was transcribed from the early to late stages of infection and the transcripts were seen from 6 to 72 h post infection (hpi). The early transcripts initiated from a consensus CAGT motif while the late transcripts arose from three conserved TAAG motifs, all of which were located in the near upstream region of the coding sequence. Both early and late transcripts terminated at a run of T residues following the second polyadenylation signal located 31 nt downstream of the translation termination codon. BmGP64 protein was detectable from 6 hpi and was present in larger quantities throughout the infection process from 12 hpi, in BmNPV-infected BmN cells. The persistent presence of GP64 in BmN cells differed from the protein expression pattern of GP64 in Autographa californica multinucleocapsid nucleopolyhedrovirus infection, where the protein levels decreased significantly by late times (48 hpi). BmGP64 was located in the membrane and cytoplasm of the infected host cells and as a component of the budded virions. The production of infectious budded virus and the fusion activity were reduced when glycosylation of GP64 was inhibited.

Original languageEnglish (US)
Pages (from-to)45-57
Number of pages13
JournalVirus research
Volume94
Issue number1
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

Keywords

  • Baculoviruses
  • BmNPV
  • Cell fusion
  • Gene expression profiling
  • Silkworms
  • Transcriptional regulation

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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