@article{89807ecfd070465ea366d19dd85305de,
title = "Characterization of RNA with unusual electrophoretic mobility from tissues of patients with Crohn's disease",
abstract = "RNAs from tissues of patients with Crohn's disease that migrate off the diagonal in a two-dimensional gel electrophoresis system were partially characterized. One of the RNA species was a discrete cleavage product of region V2-9 of 28S rRNA; another is a conformer or variant of 5.8S rRNA; and a third is a mixture of unidentified fragments with mobility similar to that of 7S RNA. The yield of these species from resected tissue and their visualization by silver staining was very sensitive to the details of the preparative procedure. No evidence of viroid-like RNA was found within the range of molecular sizes (<7S) that we examined.",
keywords = "Cell death, Endoribonuclease, Human intestinal tissue, rRNA, 28S",
author = "Urban Lundberg and West, {A. Brian} and Sidney Altman",
note = "Funding Information: Acknowledgments. The authors thank Dr. Cecilia Guerner-Takada and Donna Wesolowski for assistance with some experiments and Dr JeanPierre Perrault for adVice on preparative techniques. Drs. Allan Goldberg and Hugh Robertson for stimulatmg our interest m Crohn's Disease. and Dr. Henry Bmder for encouragement. Dr. Kenneth Batts (Mayo Clime. Rochester. MN) generously proVided many of the samples of resected tissue used in thiS study. In addition. Dr. R Petras (Cleveland Climc). the Shriner Burns Chnic (Mass. General Hospital) and Dr. Alfred Fish (Umversity ofMmnesota Medical School) supphed tissue of the early stages of this study. U.L. was supported by a fellowship from the Wenner-Gren Center Foundation for SCientific Research (Stockholm) ThiS research was supported by a grant from the Crohn's and Colitis Foundation of America and by a grant (GM 19422) from USPHS to SA.",
year = "1995",
month = sep,
day = "11",
doi = "10.1016/0014-5793(95)00946-7",
language = "English (US)",
volume = "371",
pages = "345--350",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",
}