Characterization of RNA with unusual electrophoretic mobility from tissues of patients with Crohn's disease

Urban Lundberg, A. Brian West, Sidney Altman

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

RNAs from tissues of patients with Crohn's disease that migrate off the diagonal in a two-dimensional gel electrophoresis system were partially characterized. One of the RNA species was a discrete cleavage product of region V2-9 of 28S rRNA; another is a conformer or variant of 5.8S rRNA; and a third is a mixture of unidentified fragments with mobility similar to that of 7S RNA. The yield of these species from resected tissue and their visualization by silver staining was very sensitive to the details of the preparative procedure. No evidence of viroid-like RNA was found within the range of molecular sizes (<7S) that we examined.

Original languageEnglish (US)
Pages (from-to)345-350
Number of pages6
JournalFEBS Letters
Volume371
Issue number3
DOIs
StatePublished - Sep 11 1995
Externally publishedYes

Fingerprint

Electrophoretic mobility
Crohn Disease
RNA
Tissue
Viroids
Silver Staining
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Silver
Visualization
Gels

Keywords

  • Cell death
  • Endoribonuclease
  • Human intestinal tissue
  • rRNA, 28S

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Characterization of RNA with unusual electrophoretic mobility from tissues of patients with Crohn's disease. / Lundberg, Urban; West, A. Brian; Altman, Sidney.

In: FEBS Letters, Vol. 371, No. 3, 11.09.1995, p. 345-350.

Research output: Contribution to journalArticle

@article{89807ecfd070465ea366d19dd85305de,
title = "Characterization of RNA with unusual electrophoretic mobility from tissues of patients with Crohn's disease",
abstract = "RNAs from tissues of patients with Crohn's disease that migrate off the diagonal in a two-dimensional gel electrophoresis system were partially characterized. One of the RNA species was a discrete cleavage product of region V2-9 of 28S rRNA; another is a conformer or variant of 5.8S rRNA; and a third is a mixture of unidentified fragments with mobility similar to that of 7S RNA. The yield of these species from resected tissue and their visualization by silver staining was very sensitive to the details of the preparative procedure. No evidence of viroid-like RNA was found within the range of molecular sizes (<7S) that we examined.",
keywords = "Cell death, Endoribonuclease, Human intestinal tissue, rRNA, 28S",
author = "Urban Lundberg and West, {A. Brian} and Sidney Altman",
year = "1995",
month = "9",
day = "11",
doi = "10.1016/0014-5793(95)00946-7",
language = "English (US)",
volume = "371",
pages = "345--350",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Characterization of RNA with unusual electrophoretic mobility from tissues of patients with Crohn's disease

AU - Lundberg, Urban

AU - West, A. Brian

AU - Altman, Sidney

PY - 1995/9/11

Y1 - 1995/9/11

N2 - RNAs from tissues of patients with Crohn's disease that migrate off the diagonal in a two-dimensional gel electrophoresis system were partially characterized. One of the RNA species was a discrete cleavage product of region V2-9 of 28S rRNA; another is a conformer or variant of 5.8S rRNA; and a third is a mixture of unidentified fragments with mobility similar to that of 7S RNA. The yield of these species from resected tissue and their visualization by silver staining was very sensitive to the details of the preparative procedure. No evidence of viroid-like RNA was found within the range of molecular sizes (<7S) that we examined.

AB - RNAs from tissues of patients with Crohn's disease that migrate off the diagonal in a two-dimensional gel electrophoresis system were partially characterized. One of the RNA species was a discrete cleavage product of region V2-9 of 28S rRNA; another is a conformer or variant of 5.8S rRNA; and a third is a mixture of unidentified fragments with mobility similar to that of 7S RNA. The yield of these species from resected tissue and their visualization by silver staining was very sensitive to the details of the preparative procedure. No evidence of viroid-like RNA was found within the range of molecular sizes (<7S) that we examined.

KW - Cell death

KW - Endoribonuclease

KW - Human intestinal tissue

KW - rRNA, 28S

UR - http://www.scopus.com/inward/record.url?scp=0029160309&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029160309&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(95)00946-7

DO - 10.1016/0014-5793(95)00946-7

M3 - Article

VL - 371

SP - 345

EP - 350

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -