Characterization of ribonuclease NU cleavage sites in a bacteriophage phi80 induced ribonucleic acid

A. L.M. Bothwell, S. Altman

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Ribonuclease NU, an endoribonuclease isolated from human KB tissue culture cells, can cleave a bacteriophage phi 80 induced RNA at 4 distinct sites. Nucleotide sequence analysis of the 8 cleavage products has shown that the enzyme produces oligonucleotides terminating in 3' phosphate groups, and that the 4 cleavage sites are in the only nonhydrogen bonded region of the substrate. Various aspects of the cleavage reaction with this RNA and with other substrates are discussed.

Original languageEnglish (US)
Pages (from-to)1460-1463
Number of pages4
JournalJournal of Biological Chemistry
Volume250
Issue number4
StatePublished - Dec 1 1975
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Characterization of ribonuclease NU cleavage sites in a bacteriophage phi80 induced ribonucleic acid'. Together they form a unique fingerprint.

  • Cite this