Characterization of Protein Nanocrystals Based on the Reversibility of Crystallization

Katerina Dörner, Jose M. Martin-Garcia, Christopher Kupitz, Zhen Gong, T. Conn Mallet, Liqing Chen, Rebekka Wachter, Petra Fromme

Research output: Contribution to journalArticle

Abstract

A new approach is described to screen for protein nanocrystals based on the reversibility of crystallization. Methods to characterize nanocrystals are in strong need to facilitate sample preparation for serial femtosecond X-ray nanocrystallography (SFX). SFX enables protein structure determination by collecting X-ray diffraction from nano- and microcrystals using a free electron laser. This technique is especially valuable for challenging proteins as for example membrane proteins and is in general a powerful method to overcome the radiation damage problem and to perform time-resolved structure analysis. Nanocrystal growth cannot be monitored with common methods used in protein crystallography, as the resolution of bright field microscopy is not sufficient. A high-performance method to screen for nanocrystals is second order nonlinear imaging of chiral crystals (SONICC). However, the high cost prevents its use in every laboratory, and some protein nanocrystals may be "invisible" to SONICC. In this work using a crystallization robot and a common imaging system precipitation comprised of nanocrystals and precipitation caused by aggregated protein can be distinguished.

Original languageEnglish (US)
Pages (from-to)3838-3845
Number of pages8
JournalCrystal Growth and Design
Volume16
Issue number7
DOIs
StatePublished - Jul 6 2016

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Crystallization
Nanocrystals
nanocrystals
crystallization
proteins
Proteins
Imaging techniques
X rays
Crystals
Microcrystals
x rays
Crystallography
Free electron lasers
microcrystals
Radiation damage
robots
radiation damage
free electron lasers
Imaging systems
crystallography

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Dörner, K., Martin-Garcia, J. M., Kupitz, C., Gong, Z., Mallet, T. C., Chen, L., ... Fromme, P. (2016). Characterization of Protein Nanocrystals Based on the Reversibility of Crystallization. Crystal Growth and Design, 16(7), 3838-3845. https://doi.org/10.1021/acs.cgd.6b00384

Characterization of Protein Nanocrystals Based on the Reversibility of Crystallization. / Dörner, Katerina; Martin-Garcia, Jose M.; Kupitz, Christopher; Gong, Zhen; Mallet, T. Conn; Chen, Liqing; Wachter, Rebekka; Fromme, Petra.

In: Crystal Growth and Design, Vol. 16, No. 7, 06.07.2016, p. 3838-3845.

Research output: Contribution to journalArticle

Dörner K, Martin-Garcia JM, Kupitz C, Gong Z, Mallet TC, Chen L et al. Characterization of Protein Nanocrystals Based on the Reversibility of Crystallization. Crystal Growth and Design. 2016 Jul 6;16(7):3838-3845. https://doi.org/10.1021/acs.cgd.6b00384
Dörner, Katerina ; Martin-Garcia, Jose M. ; Kupitz, Christopher ; Gong, Zhen ; Mallet, T. Conn ; Chen, Liqing ; Wachter, Rebekka ; Fromme, Petra. / Characterization of Protein Nanocrystals Based on the Reversibility of Crystallization. In: Crystal Growth and Design. 2016 ; Vol. 16, No. 7. pp. 3838-3845.
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