Abstract

Here we present a scheme to separate particles according to their characteristic physical properties, including size, charge, polarizability, deformability, surface charge mobility, dielectric features, and local capacitance. Separation is accomplished using a microdevice based on direct current insulator gradient dielectrophoresis that can isolate and concentrate multiple analytes simultaneously at different positions. The device is dependent upon dielectrophoretic and electrokinetic forces incorporating a global longitudinal direct current field as well as using shaped insulating features within the channel to induce local gradients. This design allows for the production of strong local field gradients along a global field causing particles to enter, initially transported through the channel by electrophoresis and electroosmosis (electrokinetics), and to be isolated via repulsive dielectrophoretic forces that are proportional to an exponent of the field gradient. Sulfate-capped polystyrene nano and microparticles (20, 200-nm, and 1-μm) were used as probes to demonstrate the influence of channel geometry and applied longitudinal field on separation behavior. These results are consistent with models using similar channel geometry and indicate that specific particulate species can be isolated within a distinct portion of the device, whereas concentrating particles by factors from 103 to 106.

Original languageEnglish (US)
Pages (from-to)3634-3641
Number of pages8
JournalElectrophoresis
Volume31
Issue number22
DOIs
StatePublished - Nov 2010

Fingerprint

Lab-On-A-Chip Devices
Electrophoresis
Microfluidics
Electroosmosis
Equipment and Supplies
Geometry
Polystyrenes
Surface charge
Formability
Sulfates
Capacitance
Physical properties

Keywords

  • Dielectrophoresis
  • Particle trapping
  • Separation

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Characterization of particle capture in a sawtooth patterned insulating electrokinetic microfluidic device. / Staton, Sarah J R; Chen, Kangping; Taylor, Thomas; Pacheco, Jose Rafael; Hayes, Mark.

In: Electrophoresis, Vol. 31, No. 22, 11.2010, p. 3634-3641.

Research output: Contribution to journalArticle

@article{28b0817d4e694b48827ee02f347ab17c,
title = "Characterization of particle capture in a sawtooth patterned insulating electrokinetic microfluidic device",
abstract = "Here we present a scheme to separate particles according to their characteristic physical properties, including size, charge, polarizability, deformability, surface charge mobility, dielectric features, and local capacitance. Separation is accomplished using a microdevice based on direct current insulator gradient dielectrophoresis that can isolate and concentrate multiple analytes simultaneously at different positions. The device is dependent upon dielectrophoretic and electrokinetic forces incorporating a global longitudinal direct current field as well as using shaped insulating features within the channel to induce local gradients. This design allows for the production of strong local field gradients along a global field causing particles to enter, initially transported through the channel by electrophoresis and electroosmosis (electrokinetics), and to be isolated via repulsive dielectrophoretic forces that are proportional to an exponent of the field gradient. Sulfate-capped polystyrene nano and microparticles (20, 200-nm, and 1-μm) were used as probes to demonstrate the influence of channel geometry and applied longitudinal field on separation behavior. These results are consistent with models using similar channel geometry and indicate that specific particulate species can be isolated within a distinct portion of the device, whereas concentrating particles by factors from 103 to 106.",
keywords = "Dielectrophoresis, Particle trapping, Separation",
author = "Staton, {Sarah J R} and Kangping Chen and Thomas Taylor and Pacheco, {Jose Rafael} and Mark Hayes",
year = "2010",
month = "11",
doi = "10.1002/elps.201000438",
language = "English (US)",
volume = "31",
pages = "3634--3641",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "22",

}

TY - JOUR

T1 - Characterization of particle capture in a sawtooth patterned insulating electrokinetic microfluidic device

AU - Staton, Sarah J R

AU - Chen, Kangping

AU - Taylor, Thomas

AU - Pacheco, Jose Rafael

AU - Hayes, Mark

PY - 2010/11

Y1 - 2010/11

N2 - Here we present a scheme to separate particles according to their characteristic physical properties, including size, charge, polarizability, deformability, surface charge mobility, dielectric features, and local capacitance. Separation is accomplished using a microdevice based on direct current insulator gradient dielectrophoresis that can isolate and concentrate multiple analytes simultaneously at different positions. The device is dependent upon dielectrophoretic and electrokinetic forces incorporating a global longitudinal direct current field as well as using shaped insulating features within the channel to induce local gradients. This design allows for the production of strong local field gradients along a global field causing particles to enter, initially transported through the channel by electrophoresis and electroosmosis (electrokinetics), and to be isolated via repulsive dielectrophoretic forces that are proportional to an exponent of the field gradient. Sulfate-capped polystyrene nano and microparticles (20, 200-nm, and 1-μm) were used as probes to demonstrate the influence of channel geometry and applied longitudinal field on separation behavior. These results are consistent with models using similar channel geometry and indicate that specific particulate species can be isolated within a distinct portion of the device, whereas concentrating particles by factors from 103 to 106.

AB - Here we present a scheme to separate particles according to their characteristic physical properties, including size, charge, polarizability, deformability, surface charge mobility, dielectric features, and local capacitance. Separation is accomplished using a microdevice based on direct current insulator gradient dielectrophoresis that can isolate and concentrate multiple analytes simultaneously at different positions. The device is dependent upon dielectrophoretic and electrokinetic forces incorporating a global longitudinal direct current field as well as using shaped insulating features within the channel to induce local gradients. This design allows for the production of strong local field gradients along a global field causing particles to enter, initially transported through the channel by electrophoresis and electroosmosis (electrokinetics), and to be isolated via repulsive dielectrophoretic forces that are proportional to an exponent of the field gradient. Sulfate-capped polystyrene nano and microparticles (20, 200-nm, and 1-μm) were used as probes to demonstrate the influence of channel geometry and applied longitudinal field on separation behavior. These results are consistent with models using similar channel geometry and indicate that specific particulate species can be isolated within a distinct portion of the device, whereas concentrating particles by factors from 103 to 106.

KW - Dielectrophoresis

KW - Particle trapping

KW - Separation

UR - http://www.scopus.com/inward/record.url?scp=78449258028&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78449258028&partnerID=8YFLogxK

U2 - 10.1002/elps.201000438

DO - 10.1002/elps.201000438

M3 - Article

VL - 31

SP - 3634

EP - 3641

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 22

ER -