Characterization of Ligands of a High-Affinity Metal-Binding Site in the Latent Chloroplast F1-ATPase by EPR Spectroscopy of Bound VO2+

Andrew L.P. Houseman, Lola Morgan, Russell LoBrutto, Wayne Frasch

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Vanadyl, (V=O)2+, is able to substitute for Mg2+ as a cofactor for ATPase activity catalyzed by the chloroplast F1-ATPase (CF1). Mg2+-dependent ATPase activity was also observed with CFi that contained VO2+-ATP bound specifically to the noncatalytic N2 site. Modulation of the Mg2+-ATPase activity induced by VO2+ bound at this site indicates that the metal bound to the noncatalytic site affects catalytic activity. When CF1 is depleted of nucleotides from all but the N1 site, a single Mg2+ remains bound at a site designated Ml. Addition of VO2+ to the depleted protein gives rise to an EPR spectrum characteristic of a CF1-bound VO2+ species. The binding curve of the VO2+ complex to latent, nucleotide-depleted CFi was determined by the integrated intensities of the —5/2∥ peak in the EPR spectrum as calibrated using atomic absorption spectroscopy. Under these conditions, VO2+ binds cooperatively to approximately two sites designated M2 and M3. Three-pulse ESEEM spectra of the CF1-VO2+ complex contain two intense modulations with frequencies and field-dependent behavior that show that they are from a directly coordinated 14N nucleus. Analysis of the bound VO2+ by ENDOR spectroscopy revealed the presence of a single group of protons associated with an equatorial amino or water ligand that is exchangeable with solvent. Using the additivity relation for hyperfine coupling, the most probable set of equatorial ligands to the VO2+ bound to CFi under these conditions consists of one lysine nitrogen, two carboxyl oxygens from aspartate or glutamate, and one water.

Original languageEnglish (US)
Pages (from-to)4910-4917
Number of pages8
JournalBiochemistry
Volume33
Issue number16
DOIs
StatePublished - Apr 1 1994

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Chloroplast Proton-Translocating ATPases
Proton-Translocating ATPases
Paramagnetic resonance
Spectrum Analysis
Metals
Binding Sites
Spectroscopy
Ligands
Ca(2+) Mg(2+)-ATPase
Nucleotides
Modulation
Atomic spectroscopy
Vanadates
Water
Electron Spin Resonance Spectroscopy
Absorption spectroscopy
Aspartic Acid
Lysine
Adenosine Triphosphatases
Protons

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of Ligands of a High-Affinity Metal-Binding Site in the Latent Chloroplast F1-ATPase by EPR Spectroscopy of Bound VO2+. / Houseman, Andrew L.P.; Morgan, Lola; LoBrutto, Russell; Frasch, Wayne.

In: Biochemistry, Vol. 33, No. 16, 01.04.1994, p. 4910-4917.

Research output: Contribution to journalArticle

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abstract = "Vanadyl, (V=O)2+, is able to substitute for Mg2+ as a cofactor for ATPase activity catalyzed by the chloroplast F1-ATPase (CF1). Mg2+-dependent ATPase activity was also observed with CFi that contained VO2+-ATP bound specifically to the noncatalytic N2 site. Modulation of the Mg2+-ATPase activity induced by VO2+ bound at this site indicates that the metal bound to the noncatalytic site affects catalytic activity. When CF1 is depleted of nucleotides from all but the N1 site, a single Mg2+ remains bound at a site designated Ml. Addition of VO2+ to the depleted protein gives rise to an EPR spectrum characteristic of a CF1-bound VO2+ species. The binding curve of the VO2+ complex to latent, nucleotide-depleted CFi was determined by the integrated intensities of the —5/2∥ peak in the EPR spectrum as calibrated using atomic absorption spectroscopy. Under these conditions, VO2+ binds cooperatively to approximately two sites designated M2 and M3. Three-pulse ESEEM spectra of the CF1-VO2+ complex contain two intense modulations with frequencies and field-dependent behavior that show that they are from a directly coordinated 14N nucleus. Analysis of the bound VO2+ by ENDOR spectroscopy revealed the presence of a single group of protons associated with an equatorial amino or water ligand that is exchangeable with solvent. Using the additivity relation for hyperfine coupling, the most probable set of equatorial ligands to the VO2+ bound to CFi under these conditions consists of one lysine nitrogen, two carboxyl oxygens from aspartate or glutamate, and one water.",
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N2 - Vanadyl, (V=O)2+, is able to substitute for Mg2+ as a cofactor for ATPase activity catalyzed by the chloroplast F1-ATPase (CF1). Mg2+-dependent ATPase activity was also observed with CFi that contained VO2+-ATP bound specifically to the noncatalytic N2 site. Modulation of the Mg2+-ATPase activity induced by VO2+ bound at this site indicates that the metal bound to the noncatalytic site affects catalytic activity. When CF1 is depleted of nucleotides from all but the N1 site, a single Mg2+ remains bound at a site designated Ml. Addition of VO2+ to the depleted protein gives rise to an EPR spectrum characteristic of a CF1-bound VO2+ species. The binding curve of the VO2+ complex to latent, nucleotide-depleted CFi was determined by the integrated intensities of the —5/2∥ peak in the EPR spectrum as calibrated using atomic absorption spectroscopy. Under these conditions, VO2+ binds cooperatively to approximately two sites designated M2 and M3. Three-pulse ESEEM spectra of the CF1-VO2+ complex contain two intense modulations with frequencies and field-dependent behavior that show that they are from a directly coordinated 14N nucleus. Analysis of the bound VO2+ by ENDOR spectroscopy revealed the presence of a single group of protons associated with an equatorial amino or water ligand that is exchangeable with solvent. Using the additivity relation for hyperfine coupling, the most probable set of equatorial ligands to the VO2+ bound to CFi under these conditions consists of one lysine nitrogen, two carboxyl oxygens from aspartate or glutamate, and one water.

AB - Vanadyl, (V=O)2+, is able to substitute for Mg2+ as a cofactor for ATPase activity catalyzed by the chloroplast F1-ATPase (CF1). Mg2+-dependent ATPase activity was also observed with CFi that contained VO2+-ATP bound specifically to the noncatalytic N2 site. Modulation of the Mg2+-ATPase activity induced by VO2+ bound at this site indicates that the metal bound to the noncatalytic site affects catalytic activity. When CF1 is depleted of nucleotides from all but the N1 site, a single Mg2+ remains bound at a site designated Ml. Addition of VO2+ to the depleted protein gives rise to an EPR spectrum characteristic of a CF1-bound VO2+ species. The binding curve of the VO2+ complex to latent, nucleotide-depleted CFi was determined by the integrated intensities of the —5/2∥ peak in the EPR spectrum as calibrated using atomic absorption spectroscopy. Under these conditions, VO2+ binds cooperatively to approximately two sites designated M2 and M3. Three-pulse ESEEM spectra of the CF1-VO2+ complex contain two intense modulations with frequencies and field-dependent behavior that show that they are from a directly coordinated 14N nucleus. Analysis of the bound VO2+ by ENDOR spectroscopy revealed the presence of a single group of protons associated with an equatorial amino or water ligand that is exchangeable with solvent. Using the additivity relation for hyperfine coupling, the most probable set of equatorial ligands to the VO2+ bound to CFi under these conditions consists of one lysine nitrogen, two carboxyl oxygens from aspartate or glutamate, and one water.

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