Characterization of cytoplasmic mutants of Nicotiana tabacum with altered photosynthetic function

Catherine P. Chia, John H. Duesing, Janet L. Watson, Rachel Guy, C. J. Arntzen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A series of cytoplasmic mutants of tobacco (Nicotiana tabacum) were generated and characterized. Compared to wild type tobacco, they were found to have diminished levels of photosynthetic pigments and a range of functional impairments including modified chlorophyll fluorescence properties, loss of Photosystem I and/or II electron transport activity, and aberrant ultrastructure. Although the loss of defined functional activities was correlated with the depletion of specific thylakoid membrane proteins, no simple rules governed the relationship between structural defects and photosynthetic deficiencies. All of these mutants exhibited pleiotropic losses of polypeptides, including those known to be nuclear-encoded; this is consistent with the concept that loss of one component of a multi-subunit membrane protein complex results in unstable complex assembly. The phenotype of two mutants was developmentally regulated, in one case with slow chloroplast developments and in the other by premature senescence of Photosystem II centers as a function of leaf development. These mutants should be especially useful in studying membrane protein assembly.

Original languageEnglish (US)
Pages (from-to)469-479
Number of pages11
JournalCurrent Genetics
Volume10
Issue number6
DOIs
StatePublished - Feb 1986
Externally publishedYes

Fingerprint

Tobacco
Photosystem II Protein Complex
Thylakoid Membrane Proteins
Membrane Proteins
Photosystem I Protein Complex
Chloroplasts
Chlorophyll
Electron Transport
Fluorescence
Phenotype
Peptides

Keywords

  • Chloroplasts
  • Membrane protein complexes
  • Photosynthetic mutants

ASJC Scopus subject areas

  • Genetics

Cite this

Chia, C. P., Duesing, J. H., Watson, J. L., Guy, R., & Arntzen, C. J. (1986). Characterization of cytoplasmic mutants of Nicotiana tabacum with altered photosynthetic function. Current Genetics, 10(6), 469-479. https://doi.org/10.1007/BF00419876

Characterization of cytoplasmic mutants of Nicotiana tabacum with altered photosynthetic function. / Chia, Catherine P.; Duesing, John H.; Watson, Janet L.; Guy, Rachel; Arntzen, C. J.

In: Current Genetics, Vol. 10, No. 6, 02.1986, p. 469-479.

Research output: Contribution to journalArticle

Chia, Catherine P. ; Duesing, John H. ; Watson, Janet L. ; Guy, Rachel ; Arntzen, C. J. / Characterization of cytoplasmic mutants of Nicotiana tabacum with altered photosynthetic function. In: Current Genetics. 1986 ; Vol. 10, No. 6. pp. 469-479.
@article{cd8b1775102a4157b58af2acfe2b019f,
title = "Characterization of cytoplasmic mutants of Nicotiana tabacum with altered photosynthetic function",
abstract = "A series of cytoplasmic mutants of tobacco (Nicotiana tabacum) were generated and characterized. Compared to wild type tobacco, they were found to have diminished levels of photosynthetic pigments and a range of functional impairments including modified chlorophyll fluorescence properties, loss of Photosystem I and/or II electron transport activity, and aberrant ultrastructure. Although the loss of defined functional activities was correlated with the depletion of specific thylakoid membrane proteins, no simple rules governed the relationship between structural defects and photosynthetic deficiencies. All of these mutants exhibited pleiotropic losses of polypeptides, including those known to be nuclear-encoded; this is consistent with the concept that loss of one component of a multi-subunit membrane protein complex results in unstable complex assembly. The phenotype of two mutants was developmentally regulated, in one case with slow chloroplast developments and in the other by premature senescence of Photosystem II centers as a function of leaf development. These mutants should be especially useful in studying membrane protein assembly.",
keywords = "Chloroplasts, Membrane protein complexes, Photosynthetic mutants",
author = "Chia, {Catherine P.} and Duesing, {John H.} and Watson, {Janet L.} and Rachel Guy and Arntzen, {C. J.}",
year = "1986",
month = "2",
doi = "10.1007/BF00419876",
language = "English (US)",
volume = "10",
pages = "469--479",
journal = "Current Genetics",
issn = "0172-8083",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Characterization of cytoplasmic mutants of Nicotiana tabacum with altered photosynthetic function

AU - Chia, Catherine P.

AU - Duesing, John H.

AU - Watson, Janet L.

AU - Guy, Rachel

AU - Arntzen, C. J.

PY - 1986/2

Y1 - 1986/2

N2 - A series of cytoplasmic mutants of tobacco (Nicotiana tabacum) were generated and characterized. Compared to wild type tobacco, they were found to have diminished levels of photosynthetic pigments and a range of functional impairments including modified chlorophyll fluorescence properties, loss of Photosystem I and/or II electron transport activity, and aberrant ultrastructure. Although the loss of defined functional activities was correlated with the depletion of specific thylakoid membrane proteins, no simple rules governed the relationship between structural defects and photosynthetic deficiencies. All of these mutants exhibited pleiotropic losses of polypeptides, including those known to be nuclear-encoded; this is consistent with the concept that loss of one component of a multi-subunit membrane protein complex results in unstable complex assembly. The phenotype of two mutants was developmentally regulated, in one case with slow chloroplast developments and in the other by premature senescence of Photosystem II centers as a function of leaf development. These mutants should be especially useful in studying membrane protein assembly.

AB - A series of cytoplasmic mutants of tobacco (Nicotiana tabacum) were generated and characterized. Compared to wild type tobacco, they were found to have diminished levels of photosynthetic pigments and a range of functional impairments including modified chlorophyll fluorescence properties, loss of Photosystem I and/or II electron transport activity, and aberrant ultrastructure. Although the loss of defined functional activities was correlated with the depletion of specific thylakoid membrane proteins, no simple rules governed the relationship between structural defects and photosynthetic deficiencies. All of these mutants exhibited pleiotropic losses of polypeptides, including those known to be nuclear-encoded; this is consistent with the concept that loss of one component of a multi-subunit membrane protein complex results in unstable complex assembly. The phenotype of two mutants was developmentally regulated, in one case with slow chloroplast developments and in the other by premature senescence of Photosystem II centers as a function of leaf development. These mutants should be especially useful in studying membrane protein assembly.

KW - Chloroplasts

KW - Membrane protein complexes

KW - Photosynthetic mutants

UR - http://www.scopus.com/inward/record.url?scp=34250125598&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250125598&partnerID=8YFLogxK

U2 - 10.1007/BF00419876

DO - 10.1007/BF00419876

M3 - Article

VL - 10

SP - 469

EP - 479

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

IS - 6

ER -