Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates

Andreza H. Vidal; Cristiano Lacorte; Marcio M. Sanches; Dione M.T. Alves-Freitas; Emanuel F.M. Abreu; Bruna Pinheiro-Lima; Raul C.Carriello Rosa; Onildo N. Jesus; Magnólia A. Campos; Gustavo P. Felix; Ana Clara R. Abreu; Yam S. Santos; Ana Luiza M. Lacerda; Arvind Varsani; Fernando L. Melo; Simone G. Ribeiro

doi:10.3390/v15020410

Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates

Andreza H. Vidal, Cristiano Lacorte, Marcio M. Sanches, Dione M.T. Alves-Freitas, Emanuel F.M. Abreu, Bruna Pinheiro-Lima, Raul C.Carriello Rosa, Onildo N. Jesus, Magnólia A. Campos, Gustavo P. Felix, Ana Clara R. Abreu, Yam S. Santos, Ana Luiza M. Lacerda, Arvind Varsani, Fernando L. Melo, Simone G. Ribeiro

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

High-throughput sequencing (HTS) has been an important tool for the discovery of plant viruses and their surveillance. In 2015, several virus-like symptoms were observed in passion fruit (PF) plants in Bahia state, Brazil. Using HTS technology, bioinformatics tools, RT-PCR, and Sanger sequencing, we identified the cucurbit aphid-borne yellows virus (CABYV, Polerovirus, Solemoviridae) in co-infection with cowpea aphid-borne mosaic virus (CABMV, Potyvirus, Potyviridae) in PF, in green manure, and spontaneous plants in several localities in Bahia. Complete genomes of CABYV-PF isolates were determined and analyzed with other CABYV isolates available in GenBank that have been identified in various countries. Phylogenetic analysis and pairwise identity comparison with CABYV isolates showed that CABYV-PFs are more closely related to French and Spanish isolates. Overall, analyses of all the CABYV genomes revealed that these could represent ten distinct species, and we thus proposed reclassifying these CABYV as isolates into ten species, tentatively named “Polerovirus curcubitaeprimum” to “Polerovirus curcubitaenonum”, and “Polerovirus melo”. CABYV-PF is a member of “Polerovirus curcubitaeprimum”.

Original language	English (US)
Article number	410
Journal	Viruses
Volume	15
Issue number	2
DOIs	https://doi.org/10.3390/v15020410
State	Published - Feb 2023

Keywords

CABMV
CABYV
Passiflora
mixed infection
polerovirus

ASJC Scopus subject areas

Infectious Diseases
Virology

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10.3390/v15020410

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Vidal, A. H., Lacorte, C., Sanches, M. M., Alves-Freitas, D. M. T., Abreu, E. F. M., Pinheiro-Lima, B., Rosa, R. C. C., Jesus, O. N., Campos, M. A., Felix, G. P., Abreu, A. C. R., Santos, Y. S., Lacerda, A. L. M., Varsani, A., Melo, F. L., & Ribeiro, S. G. (2023). Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates. Viruses, 15(2), Article 410. https://doi.org/10.3390/v15020410

Vidal, AH, Lacorte, C, Sanches, MM, Alves-Freitas, DMT, Abreu, EFM, Pinheiro-Lima, B, Rosa, RCC, Jesus, ON, Campos, MA, Felix, GP, Abreu, ACR, Santos, YS, Lacerda, ALM, Varsani, A, Melo, FL & Ribeiro, SG 2023, 'Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates', Viruses, vol. 15, no. 2, 410. https://doi.org/10.3390/v15020410

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title = "Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates",

abstract = "High-throughput sequencing (HTS) has been an important tool for the discovery of plant viruses and their surveillance. In 2015, several virus-like symptoms were observed in passion fruit (PF) plants in Bahia state, Brazil. Using HTS technology, bioinformatics tools, RT-PCR, and Sanger sequencing, we identified the cucurbit aphid-borne yellows virus (CABYV, Polerovirus, Solemoviridae) in co-infection with cowpea aphid-borne mosaic virus (CABMV, Potyvirus, Potyviridae) in PF, in green manure, and spontaneous plants in several localities in Bahia. Complete genomes of CABYV-PF isolates were determined and analyzed with other CABYV isolates available in GenBank that have been identified in various countries. Phylogenetic analysis and pairwise identity comparison with CABYV isolates showed that CABYV-PFs are more closely related to French and Spanish isolates. Overall, analyses of all the CABYV genomes revealed that these could represent ten distinct species, and we thus proposed reclassifying these CABYV as isolates into ten species, tentatively named “Polerovirus curcubitaeprimum” to “Polerovirus curcubitaenonum”, and “Polerovirus melo”. CABYV-PF is a member of “Polerovirus curcubitaeprimum”.",

keywords = "CABMV, CABYV, Passiflora, mixed infection, polerovirus",

author = "Vidal, {Andreza H.} and Cristiano Lacorte and Sanches, {Marcio M.} and Alves-Freitas, {Dione M.T.} and Abreu, {Emanuel F.M.} and Bruna Pinheiro-Lima and Rosa, {Raul C.Carriello} and Jesus, {Onildo N.} and Campos, {Magn{\'o}lia A.} and Felix, {Gustavo P.} and Abreu, {Ana Clara R.} and Santos, {Yam S.} and Lacerda, {Ana Luiza M.} and Arvind Varsani and Melo, {Fernando L.} and Ribeiro, {Simone G.}",

note = "Funding Information: This research was funded by grants from Embrapa, the Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico—CNPq, and the Funda{\c c}{\~a}o de Apoio {\`a} Pesquisa do Distrito Federal—FAPDF. The Coordena{\c c}{\~a}o de Aperfei{\c c}oamento de Pessoal de N{\'i}vel Superior—CAPES provided scholarships to A.H.V. and B.P.-L., and CNPq did so for D.M.T.A.-F., G.P.F., A.C.R.A. and A.L.M.L. Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = feb,

doi = "10.3390/v15020410",

language = "English (US)",

volume = "15",

journal = "Viruses",

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number = "2",

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T1 - Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil

T2 - Evidence of a Complex of Species within CABYV Isolates

AU - Vidal, Andreza H.

AU - Lacorte, Cristiano

AU - Sanches, Marcio M.

AU - Alves-Freitas, Dione M.T.

AU - Abreu, Emanuel F.M.

AU - Pinheiro-Lima, Bruna

AU - Rosa, Raul C.Carriello

AU - Jesus, Onildo N.

AU - Campos, Magnólia A.

AU - Felix, Gustavo P.

AU - Abreu, Ana Clara R.

AU - Santos, Yam S.

AU - Lacerda, Ana Luiza M.

AU - Varsani, Arvind

AU - Melo, Fernando L.

AU - Ribeiro, Simone G.

N1 - Funding Information: This research was funded by grants from Embrapa, the Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq, and the Fundação de Apoio à Pesquisa do Distrito Federal—FAPDF. The Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES provided scholarships to A.H.V. and B.P.-L., and CNPq did so for D.M.T.A.-F., G.P.F., A.C.R.A. and A.L.M.L. Publisher Copyright: © 2023 by the authors.

PY - 2023/2

Y1 - 2023/2

N2 - High-throughput sequencing (HTS) has been an important tool for the discovery of plant viruses and their surveillance. In 2015, several virus-like symptoms were observed in passion fruit (PF) plants in Bahia state, Brazil. Using HTS technology, bioinformatics tools, RT-PCR, and Sanger sequencing, we identified the cucurbit aphid-borne yellows virus (CABYV, Polerovirus, Solemoviridae) in co-infection with cowpea aphid-borne mosaic virus (CABMV, Potyvirus, Potyviridae) in PF, in green manure, and spontaneous plants in several localities in Bahia. Complete genomes of CABYV-PF isolates were determined and analyzed with other CABYV isolates available in GenBank that have been identified in various countries. Phylogenetic analysis and pairwise identity comparison with CABYV isolates showed that CABYV-PFs are more closely related to French and Spanish isolates. Overall, analyses of all the CABYV genomes revealed that these could represent ten distinct species, and we thus proposed reclassifying these CABYV as isolates into ten species, tentatively named “Polerovirus curcubitaeprimum” to “Polerovirus curcubitaenonum”, and “Polerovirus melo”. CABYV-PF is a member of “Polerovirus curcubitaeprimum”.

AB - High-throughput sequencing (HTS) has been an important tool for the discovery of plant viruses and their surveillance. In 2015, several virus-like symptoms were observed in passion fruit (PF) plants in Bahia state, Brazil. Using HTS technology, bioinformatics tools, RT-PCR, and Sanger sequencing, we identified the cucurbit aphid-borne yellows virus (CABYV, Polerovirus, Solemoviridae) in co-infection with cowpea aphid-borne mosaic virus (CABMV, Potyvirus, Potyviridae) in PF, in green manure, and spontaneous plants in several localities in Bahia. Complete genomes of CABYV-PF isolates were determined and analyzed with other CABYV isolates available in GenBank that have been identified in various countries. Phylogenetic analysis and pairwise identity comparison with CABYV isolates showed that CABYV-PFs are more closely related to French and Spanish isolates. Overall, analyses of all the CABYV genomes revealed that these could represent ten distinct species, and we thus proposed reclassifying these CABYV as isolates into ten species, tentatively named “Polerovirus curcubitaeprimum” to “Polerovirus curcubitaenonum”, and “Polerovirus melo”. CABYV-PF is a member of “Polerovirus curcubitaeprimum”.

KW - CABMV

KW - CABYV

KW - Passiflora

KW - mixed infection

KW - polerovirus

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ER -

Characterization of Cucurbit Aphid-Borne Yellows Virus (CABYV) from Passion Fruit in Brazil: Evidence of a Complex of Species within CABYV Isolates

Abstract

Keywords

ASJC Scopus subject areas

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