Characterization of autoantibodies directed against T cell receptors

Douglas Lake, W. J. Landsperger, R. M. Bernstein, S. F. Schluter, J. J. Marchalonis

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Recently it has been observed that administration of intravenous immunoglobulin (IVIG) can have profound effects on a wide variety of diseases related to the dysregulation of the immune system. The mechanisms which explain these activities are poorly understood. Human IVIG and various Cohn plasma fractions contain autoantibodies directed against T cell receptors (Tcr). Previous studies have shown that IVIG contains autoantibodies against T cell receptor peptides. In order to further our understanding of autoantibody specificities, a single chain Tcr (scTcr) was constructed by recombinant DNA techniques from the variable α and variable β chains of the Jurkat cell line. Anti-Tcr autoantibodies were isolated from IVIG and Cohn fractions I + III using a scTcr affinity column. This scTcr affinity purified material reacted with the surfaces oft cells at 10 μg/ml whereas non- purified IVIG did not. Sera from patients with rheumatoid arthritis (RA) as well as serum from patients with systemic lupus erythematosus (SLE) reacted with the scTcr at levels above that of normals.

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
JournalAdvances in Experimental Medicine and Biology
Volume383
StatePublished - 1995
Externally publishedYes

Fingerprint

Intravenous Immunoglobulins
T-Cell Antigen Receptor
Autoantibodies
Jurkat Cells
Recombinant DNA
Immune system
Serum
Systemic Lupus Erythematosus
Immune System
Rheumatoid Arthritis
Cells
Plasmas
Cell Line
Peptides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lake, D., Landsperger, W. J., Bernstein, R. M., Schluter, S. F., & Marchalonis, J. J. (1995). Characterization of autoantibodies directed against T cell receptors. Advances in Experimental Medicine and Biology, 383, 223-229.

Characterization of autoantibodies directed against T cell receptors. / Lake, Douglas; Landsperger, W. J.; Bernstein, R. M.; Schluter, S. F.; Marchalonis, J. J.

In: Advances in Experimental Medicine and Biology, Vol. 383, 1995, p. 223-229.

Research output: Contribution to journalArticle

Lake, D, Landsperger, WJ, Bernstein, RM, Schluter, SF & Marchalonis, JJ 1995, 'Characterization of autoantibodies directed against T cell receptors', Advances in Experimental Medicine and Biology, vol. 383, pp. 223-229.
Lake D, Landsperger WJ, Bernstein RM, Schluter SF, Marchalonis JJ. Characterization of autoantibodies directed against T cell receptors. Advances in Experimental Medicine and Biology. 1995;383:223-229.
Lake, Douglas ; Landsperger, W. J. ; Bernstein, R. M. ; Schluter, S. F. ; Marchalonis, J. J. / Characterization of autoantibodies directed against T cell receptors. In: Advances in Experimental Medicine and Biology. 1995 ; Vol. 383. pp. 223-229.
@article{b22f531e01e345fda893654cdcd778a0,
title = "Characterization of autoantibodies directed against T cell receptors",
abstract = "Recently it has been observed that administration of intravenous immunoglobulin (IVIG) can have profound effects on a wide variety of diseases related to the dysregulation of the immune system. The mechanisms which explain these activities are poorly understood. Human IVIG and various Cohn plasma fractions contain autoantibodies directed against T cell receptors (Tcr). Previous studies have shown that IVIG contains autoantibodies against T cell receptor peptides. In order to further our understanding of autoantibody specificities, a single chain Tcr (scTcr) was constructed by recombinant DNA techniques from the variable α and variable β chains of the Jurkat cell line. Anti-Tcr autoantibodies were isolated from IVIG and Cohn fractions I + III using a scTcr affinity column. This scTcr affinity purified material reacted with the surfaces oft cells at 10 μg/ml whereas non- purified IVIG did not. Sera from patients with rheumatoid arthritis (RA) as well as serum from patients with systemic lupus erythematosus (SLE) reacted with the scTcr at levels above that of normals.",
author = "Douglas Lake and Landsperger, {W. J.} and Bernstein, {R. M.} and Schluter, {S. F.} and Marchalonis, {J. J.}",
year = "1995",
language = "English (US)",
volume = "383",
pages = "223--229",
journal = "Advances in Experimental Medicine and Biology",
issn = "0065-2598",
publisher = "Springer New York",

}

TY - JOUR

T1 - Characterization of autoantibodies directed against T cell receptors

AU - Lake, Douglas

AU - Landsperger, W. J.

AU - Bernstein, R. M.

AU - Schluter, S. F.

AU - Marchalonis, J. J.

PY - 1995

Y1 - 1995

N2 - Recently it has been observed that administration of intravenous immunoglobulin (IVIG) can have profound effects on a wide variety of diseases related to the dysregulation of the immune system. The mechanisms which explain these activities are poorly understood. Human IVIG and various Cohn plasma fractions contain autoantibodies directed against T cell receptors (Tcr). Previous studies have shown that IVIG contains autoantibodies against T cell receptor peptides. In order to further our understanding of autoantibody specificities, a single chain Tcr (scTcr) was constructed by recombinant DNA techniques from the variable α and variable β chains of the Jurkat cell line. Anti-Tcr autoantibodies were isolated from IVIG and Cohn fractions I + III using a scTcr affinity column. This scTcr affinity purified material reacted with the surfaces oft cells at 10 μg/ml whereas non- purified IVIG did not. Sera from patients with rheumatoid arthritis (RA) as well as serum from patients with systemic lupus erythematosus (SLE) reacted with the scTcr at levels above that of normals.

AB - Recently it has been observed that administration of intravenous immunoglobulin (IVIG) can have profound effects on a wide variety of diseases related to the dysregulation of the immune system. The mechanisms which explain these activities are poorly understood. Human IVIG and various Cohn plasma fractions contain autoantibodies directed against T cell receptors (Tcr). Previous studies have shown that IVIG contains autoantibodies against T cell receptor peptides. In order to further our understanding of autoantibody specificities, a single chain Tcr (scTcr) was constructed by recombinant DNA techniques from the variable α and variable β chains of the Jurkat cell line. Anti-Tcr autoantibodies were isolated from IVIG and Cohn fractions I + III using a scTcr affinity column. This scTcr affinity purified material reacted with the surfaces oft cells at 10 μg/ml whereas non- purified IVIG did not. Sera from patients with rheumatoid arthritis (RA) as well as serum from patients with systemic lupus erythematosus (SLE) reacted with the scTcr at levels above that of normals.

UR - http://www.scopus.com/inward/record.url?scp=0028823372&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028823372&partnerID=8YFLogxK

M3 - Article

C2 - 8644505

AN - SCOPUS:0028823372

VL - 383

SP - 223

EP - 229

JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

SN - 0065-2598

ER -