Characterization of autoantibodies directed against T cell receptors

D. F. Lake, W. J. Landsperger, R. M. Bernstein, S. F. Schluter, J. J. Marchalonis

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Recently it has been observed that administration of intravenous immunoglobulin (IVIG) can have profound effects on a wide variety of diseases related to the dysregulation of the immune system. The mechanisms which explain these activities are poorly understood. Human IVIG and various Cohn plasma fractions contain autoantibodies directed against T cell receptors (Tcr). Previous studies have shown that IVIG contains autoantibodies against T cell receptor peptides. In order to further our understanding of autoantibody specificities, a single chain Tcr (scTcr) was constructed by recombinant DNA techniques from the variable α and variable β chains of the Jurkat cell line. Anti-Tcr autoantibodies were isolated from IVIG and Cohn fractions I + III using a scTcr affinity column. This scTcr affinity purified material reacted with the surfaces oft cells at 10 μg/ml whereas non- purified IVIG did not. Sera from patients with rheumatoid arthritis (RA) as well as serum from patients with systemic lupus erythematosus (SLE) reacted with the scTcr at levels above that of normals.

Original languageEnglish (US)
Pages (from-to)223-229
Number of pages7
JournalAdvances in experimental medicine and biology
Volume383
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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