Characterization of Alu repeats that are associated with trinucleotide and tetranucleotide repeat microsatellites

Chandri N. Yandava, Julie M. Gastier, Jacqueline C. Pulido, Tom Brody, Val Sheffield, Jeffrey Murray, Kenneth Buetow, Geoffrey M. Duyk

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The association of subclasses of Alu repetitive elements with various classes of trinucleotide and tetranucleotide microsatellites was characterized as a first step toward advancing our understanding of the evolution of microsatellite repeats. In addition, information regarding the association of specific classes of microsatellites with families of Alu elements was used to facilitate the development of genetic markers. Sequences containing Alu repeats were eliminated because unique primers could not be designed. Various classes of microsatellites are associated with different classes of Alu repeats. Very abundant and poly(A)-rich microsatellite classes (ATA, AATA) are frequently associated with an evolutionarily older subclass of Alu repeats, AluSx, whereas most of GATA and CA microsatellites are associated with a recent Alu subfamily, AluY. Our observations support all three possible mechanisms for the association of Alu repeats to microsatellites. Primers designed using a set of sequences from a particular microsatellite class showed higher homology with more sequences of that class than probes designed for other classes. We developed an efficient method of prescreening GGAA and ATA microsatellite clones for Alu repeats with probes designed in this study. We also showed that Alu probes labeled in a single reaction (multiplex labeling) could be used efficiently for prescreening of GGAA clones. Sequencing of these prescreened GGAA microsatellites revealed only 5% Alu repeats. Prescreening with primers designed for ATA microsatellite class resulted in the reduction of the loss of markers from ~50% to 10%. The new Alu probes that were designed have also proved to be useful in Alu-Alu fingerprinting.

Original languageEnglish (US)
Pages (from-to)716-724
Number of pages9
JournalGenome research
Volume7
Issue number7
DOIs
StatePublished - Jul 1997
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'Characterization of Alu repeats that are associated with trinucleotide and tetranucleotide repeat microsatellites'. Together they form a unique fingerprint.

  • Cite this

    Yandava, C. N., Gastier, J. M., Pulido, J. C., Brody, T., Sheffield, V., Murray, J., Buetow, K., & Duyk, G. M. (1997). Characterization of Alu repeats that are associated with trinucleotide and tetranucleotide repeat microsatellites. Genome research, 7(7), 716-724. https://doi.org/10.1101/gr.7.7.716