A cytopathogenic agent, designated CHV-BR, isolated from genital lesions in dogs has been shown to be a herpesvirus. It contained DNA, was ether-and heat-sensitive, and was unstable at low pH. Electron micrographs revealed a virion of typical herpesvirus structure. The CHV-BR isolate produced a marked cytopathic effect (CPE) in dog kidney cell cultures but failed to grow in rabbit kidney and human cell lines. The CPE in dog kidney cells consisted of extensive polykaryocytosis, and the formation of intranuclear type-A inclusion bodies. The emergence of a non-polykaryocytogenic CPE variant during serial passage of the isolate in dog cells was described. Neutralization and immunofluorescence tests showed that CHV-BR was serologically similar to the F-205 strain of canine herpesvirus (CHV) but was not related to other human and animal herpesviruses. Differences between the CHV-BR isolate and the CHV strains isolated elsewhere were discussed.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology