Characterization and protective properties of attenuated mutants of Salmonella choleraesuis

S. M. Kelly, B. A. Bosecker, R. Curtiss

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

We have constructed crp::Tn10 and cya::Tn10 Salmonella choleraesuis mutants and their fusaric acid-resistant derivatives with deletions (Δ) of the Tn10 and adjacent DNA sequences and found them to be avirulent and able to induce protection against a wild-type challenge in 8-week-old BALB/c mice. Mice survived infection with the crp and cya mutants at doses of more than 7 x 103 times the oral (p.o.) 50% lethal dose (LD50) and more than 8 x 102 times the intraperitoneal LD50 of the wild-type S. choleraesuis parent. Mice vaccinated with attenuated strains were protected against challenge with more than 1.6 x 104 times the p.o. LD50 and more than 80 times the intraperitoneal LD50 of the wild-type virulent S. choleraesuis parent. One deletion mutation isolated in the crp region extends to an adjacent gene(s) that was shown to be associated with avirulence. This gene or operon has been designated cdt (colonization of deep tissues). A Δ(crp-cdt)19 strain, when complemented with the wild-type crp gene and promoter on a pBR-derived plasmid, had p.o. LD50 values 103 times higher than those for the wild type. A Δcya Δ(crp-cdt)19 double mutant was less virulent than and afforded more complete protection against a challenge with the wild-type strain than a Δcrp-11 Δcya double mutant or the individual cya, crp, or crp+/cdt mutants. The deletion derivatives exhibited reduced invasion of CHO cells in vitro, and the numbers of the mutants recovered from mouse tissues were less than that of the parent strain. These studies suggest that one or more of the genes involved in cell attachment to and/or invasion of S. choleraesuis may be under catabolite repression. In addition, we describe a new deletion of a gene(s) located in the crp region between cysG and argD that is associated with virulence in S. choleraesuis.

Original languageEnglish (US)
Pages (from-to)4881-4890
Number of pages10
JournalInfection and Immunity
Volume60
Issue number11
StatePublished - 1992
Externally publishedYes

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Lethal Dose 50
Salmonella
Genes
Fusaric Acid
Catabolite Repression
CHO Cells
Sequence Deletion
Gene Deletion
Operon
Virulence
Plasmids
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Kelly, S. M., Bosecker, B. A., & Curtiss, R. (1992). Characterization and protective properties of attenuated mutants of Salmonella choleraesuis. Infection and Immunity, 60(11), 4881-4890.

Characterization and protective properties of attenuated mutants of Salmonella choleraesuis. / Kelly, S. M.; Bosecker, B. A.; Curtiss, R.

In: Infection and Immunity, Vol. 60, No. 11, 1992, p. 4881-4890.

Research output: Contribution to journalArticle

Kelly, SM, Bosecker, BA & Curtiss, R 1992, 'Characterization and protective properties of attenuated mutants of Salmonella choleraesuis', Infection and Immunity, vol. 60, no. 11, pp. 4881-4890.
Kelly, S. M. ; Bosecker, B. A. ; Curtiss, R. / Characterization and protective properties of attenuated mutants of Salmonella choleraesuis. In: Infection and Immunity. 1992 ; Vol. 60, No. 11. pp. 4881-4890.
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