Characterization and evaluation of in vitro matrix production of intervertebral disc cells

Matthew M. Holecko, S. P. Massia, V. M. Gharpuray

Research output: Contribution to journalArticlepeer-review

Abstract

The in vitro behavior of explanted intervertebral disc cells in two- and three-dimensional configurations was examined. Samples of caprine intervertebral discs were extracted from healthy animals using surgical techniques. The annulus fibrosus and nucleus pulposus were separated manually and minced. To permit cell growth in three dimensions, cells were seeded in an alginate gel suspension. After two weeks, the samples were fixed and processed using standard paraffin processing techniques. Histological sections of healthy caprine intervertebral discs were used as controls, and the cell culture samples were compared with controls in terms of cell type and density, and collagen and proteoglycan contents.

Original languageEnglish (US)
Pages (from-to)401-402
Number of pages2
JournalCritical Reviews in Biomedical Engineering
Volume26
Issue number5
StatePublished - Dec 1 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biomedical Engineering

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