TY - GEN
T1 - Characterization and application of a discrete quartz extended-gate ISFET for the assessment of tumor cell viability
AU - Arafa, Hany
AU - Obahiagbon, Uwadiae
AU - Kullman, Dixie
AU - Dominguez, Fatima Joyce
AU - Magee, Abigail
AU - Blain Christen, Jennifer
N1 - Publisher Copyright:
© 2016 IEEE.
PY - 2016/12/27
Y1 - 2016/12/27
N2 - In this work we present a system designed for continuous assessment of tumor cell extracellular pH using a fabricated quartz extended-gate ion-sensitive field effect transistor (EGFET). The extended gate structure was fabricated by patterning gold on a quartz substrate creating a pseudo-reference electrode and sensor below a Si3N4 sensing membrane. Various electrode geometries and configurations were created and each pattern was characterized. A readout/data acquisition system was designed to convert the current output of the EGFET to a voltage that was recorded using a low-power single board computer, which performed a hard 'reset' before every data acquisition interval. This setup was able to monitor the viability of SKBR3 mammary gland tumor cells treated with staurosporine. Over a span of 8 hours, the autonomous data acquisition system recorded a steady decrease in cell viability. Results were verified with periodic cell culture images. Future applications include design of an extended gate EGFET array, which allows for accurate monitoring of individual cell cultures.
AB - In this work we present a system designed for continuous assessment of tumor cell extracellular pH using a fabricated quartz extended-gate ion-sensitive field effect transistor (EGFET). The extended gate structure was fabricated by patterning gold on a quartz substrate creating a pseudo-reference electrode and sensor below a Si3N4 sensing membrane. Various electrode geometries and configurations were created and each pattern was characterized. A readout/data acquisition system was designed to convert the current output of the EGFET to a voltage that was recorded using a low-power single board computer, which performed a hard 'reset' before every data acquisition interval. This setup was able to monitor the viability of SKBR3 mammary gland tumor cells treated with staurosporine. Over a span of 8 hours, the autonomous data acquisition system recorded a steady decrease in cell viability. Results were verified with periodic cell culture images. Future applications include design of an extended gate EGFET array, which allows for accurate monitoring of individual cell cultures.
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U2 - 10.1109/HIC.2016.7797697
DO - 10.1109/HIC.2016.7797697
M3 - Conference contribution
AN - SCOPUS:85010690643
T3 - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
SP - 62
EP - 65
BT - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2016 IEEE Healthcare Innovation Point-of-Care Technologies Conference, HI-POCT 2016
Y2 - 9 November 2016 through 11 November 2016
ER -