Characteristics of bursal T lymphocytes induced by infectious bursal disease virus

I. J. Kim, S. K. You, H. Kim, H. Y. Yeh, J. M. Sharma

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Infectious bursal disease virus (IBDV) is an avian lymphotropic virus that causes immunosuppression. When specific-pathogen-free chickens were exposed to a pathogenic strain of IBDV (IM), the virus rapidly destroyed B cells in the bursa of Fabricius. Extensive viral replication was accompanied by an infiltration of T cells in the bursa. We studied the characteristics of intrabursal T lymphocytes in IBDV-infected chickens and examined whether T cells were involved in virus clearance. Flow cytometric analysis of single-cell suspensions of the bursal tissue revealed that T cells were first detectable at 4 days postinoculation (p.i.). At 7 days p.i., 65% of bursal cells were T cells and 7% were B cells. After virus infection, the numbers of bursal T cells expressing activation markers Ia and CD25 were significantly increased (P <lt> 0.03). In addition, IBDV-induced bursal T cells produced elevated levels of interleukin-6-like factor and nitric oxide-inducing factor in vitro. Spleen and bursal cells of IBDV-infected chickens had upregulated gamma interferon gene expression in comparison with virus-free chickens. In IBDV-infected chickens, bursal T cells proliferated in vitro upon stimulation with purified IBDV in a dose-dependent manner (P <lt> 0.02), whereas virus-specific T-cell expansion was not detected in the spleen. Cyclosporin A treatment, which reduced the number of circulating T cells and compromised T-cell mitogenesis, increased viral burden in the bursae of IBDV-infected chickens. The results suggest that intrabursal T cells and T-cell-mediated responses may be important in viral clearance and promoting recovery from infection.

Original languageEnglish (US)
Pages (from-to)8884-8892
Number of pages9
JournalJournal of Virology
Volume74
Issue number19
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Infectious bursal disease virus
T-lymphocytes
T-Lymphocytes
Chickens
viruses
Viruses
B-lymphocytes
spleen
B-Lymphocytes
Spleen
Bursa of Fabricius
Single-Cell Analysis
chickens
Specific Pathogen-Free Organisms
cyclosporine
bursa of Fabricius
immunosuppression
Virus Diseases
virus replication
viral load

ASJC Scopus subject areas

  • Immunology

Cite this

Characteristics of bursal T lymphocytes induced by infectious bursal disease virus. / Kim, I. J.; You, S. K.; Kim, H.; Yeh, H. Y.; Sharma, J. M.

In: Journal of Virology, Vol. 74, No. 19, 2000, p. 8884-8892.

Research output: Contribution to journalArticle

Kim, I. J. ; You, S. K. ; Kim, H. ; Yeh, H. Y. ; Sharma, J. M. / Characteristics of bursal T lymphocytes induced by infectious bursal disease virus. In: Journal of Virology. 2000 ; Vol. 74, No. 19. pp. 8884-8892.
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