Characterisation of guanidino-containing antibiotics

Field desorption mass spectrometry of bleomycin B2 and phleomycins D1 and E

Anne Dell, Howard R. Morris, Sidney Hecht, Mark D. Levin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Because of their complexity bleomycins and their relatives are difficult molecules to structurally characterise. In this paper the first successful field desorption mass spectrometric investigations of these molecules are reported. The recently corrected structure for the glycopeptide skeleton is confirmed and the preparation of a monoacetyl derivative suitable for both mass spectrometric and biological studies is described. The procedures outlined in this paper enable the unambiguous characterisation of closely related members of the bleomycin family and their derivatives, separately or in mixtures.

Original languageEnglish (US)
Pages (from-to)987-994
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume97
Issue number3
DOIs
StatePublished - Dec 16 1980
Externally publishedYes

Fingerprint

Bleomycin
Mass spectrometry
Desorption
Mass Spectrometry
Anti-Bacterial Agents
Derivatives
Molecules
Glycopeptides
Skeleton
phleomycin E
phleomycin D1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterisation of guanidino-containing antibiotics : Field desorption mass spectrometry of bleomycin B2 and phleomycins D1 and E. / Dell, Anne; Morris, Howard R.; Hecht, Sidney; Levin, Mark D.

In: Biochemical and Biophysical Research Communications, Vol. 97, No. 3, 16.12.1980, p. 987-994.

Research output: Contribution to journalArticle

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