Characterisation of guanidino-containing antibiotics: Field desorption mass spectrometry of bleomycin B2 and phleomycins D1 and E

Anne Dell; Howard R. Morris; Sidney M. Hecht; Mark D. Levin

doi:10.1016/0006-291X(80)91474-6

Characterisation of guanidino-containing antibiotics: Field desorption mass spectrometry of bleomycin B₂ and phleomycins D₁ and E

Anne Dell, Howard R. Morris, Sidney M. Hecht, Mark D. Levin

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Because of their complexity bleomycins and their relatives are difficult molecules to structurally characterise. In this paper the first successful field desorption mass spectrometric investigations of these molecules are reported. The recently corrected structure for the glycopeptide skeleton is confirmed and the preparation of a monoacetyl derivative suitable for both mass spectrometric and biological studies is described. The procedures outlined in this paper enable the unambiguous characterisation of closely related members of the bleomycin family and their derivatives, separately or in mixtures.

Original language	English (US)
Pages (from-to)	987-994
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	97
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(80)91474-6
State	Published - Dec 16 1980
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/0006-291X(80)91474-6

Cite this

@article{2e532c4b16e54b7fa900cc9ab22827ab,

title = "Characterisation of guanidino-containing antibiotics: Field desorption mass spectrometry of bleomycin B2 and phleomycins D1 and E",

abstract = "Because of their complexity bleomycins and their relatives are difficult molecules to structurally characterise. In this paper the first successful field desorption mass spectrometric investigations of these molecules are reported. The recently corrected structure for the glycopeptide skeleton is confirmed and the preparation of a monoacetyl derivative suitable for both mass spectrometric and biological studies is described. The procedures outlined in this paper enable the unambiguous characterisation of closely related members of the bleomycin family and their derivatives, separately or in mixtures.",

author = "Anne Dell and Morris, {Howard R.} and Hecht, {Sidney M.} and Levin, {Mark D.}",

note = "Funding Information: This work was supported at Imperial College Councils and at the University of Virginia by Grant the National Cancer Institute, Department of Health, The purification of bleomycins for mass spectral the doctoral program of M.D. L., -_---in absentia from",

year = "1980",

month = dec,

day = "16",

doi = "10.1016/0006-291X(80)91474-6",

language = "English (US)",

volume = "97",

pages = "987--994",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Characterisation of guanidino-containing antibiotics

T2 - Field desorption mass spectrometry of bleomycin B2 and phleomycins D1 and E

AU - Dell, Anne

AU - Morris, Howard R.

AU - Hecht, Sidney M.

AU - Levin, Mark D.

N1 - Funding Information: This work was supported at Imperial College Councils and at the University of Virginia by Grant the National Cancer Institute, Department of Health, The purification of bleomycins for mass spectral the doctoral program of M.D. L., -_---in absentia from

PY - 1980/12/16

Y1 - 1980/12/16

N2 - Because of their complexity bleomycins and their relatives are difficult molecules to structurally characterise. In this paper the first successful field desorption mass spectrometric investigations of these molecules are reported. The recently corrected structure for the glycopeptide skeleton is confirmed and the preparation of a monoacetyl derivative suitable for both mass spectrometric and biological studies is described. The procedures outlined in this paper enable the unambiguous characterisation of closely related members of the bleomycin family and their derivatives, separately or in mixtures.

AB - Because of their complexity bleomycins and their relatives are difficult molecules to structurally characterise. In this paper the first successful field desorption mass spectrometric investigations of these molecules are reported. The recently corrected structure for the glycopeptide skeleton is confirmed and the preparation of a monoacetyl derivative suitable for both mass spectrometric and biological studies is described. The procedures outlined in this paper enable the unambiguous characterisation of closely related members of the bleomycin family and their derivatives, separately or in mixtures.

UR - http://www.scopus.com/inward/record.url?scp=0019274066&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019274066&partnerID=8YFLogxK

U2 - 10.1016/0006-291X(80)91474-6

DO - 10.1016/0006-291X(80)91474-6

M3 - Article

C2 - 6162468

AN - SCOPUS:0019274066

SN - 0006-291X

VL - 97

SP - 987

EP - 994

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Characterisation of guanidino-containing antibiotics: Field desorption mass spectrometry of bleomycin B₂ and phleomycins D₁ and E

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this