Chapter 7 Monitoring Oxidative Stress in Vascular Endothelial Cells in Response to Fluid Shear Stress

From Biochemical Analyses to Micro- and Nanotechnologies

Mahsa Rouhanizadeh, Wakako Takabe, Lisong Ai, Hongyu Yu, Tzung Hsiai

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Hemodynamics, specifically, fluid shear stress, modulates the focal nature of atherosclerosis. Shear stress induces vascular oxidative stress via the activation of membrane-bound NADPH oxidases present in vascular smooth muscle cells, fibroblasts, and phagocytic mononuclear cells. Shear stress acting on the endothelial cells at arterial bifurcations or branching points regulates both NADPH oxidase and nitric oxide (NO) synthase activities. The former is considered a major source of oxygen-centered radicals (i.e., superoxide anion [O2· -]) that give rise to oxidative stress; the latter is a source of nitrogen-centered radicals (i.e., nitric oxide [NO]) that give rise to nitrative/nitrosative stress. In addition to conventional biochemical analyses, the emerging microelectromechanical systems (MEMS) provide spatial and temporal resolutions to investigate the mechanisms whereby the characteristics of shear stress regulate the biological activities of endothelial cells at the complicated arterial geometry. In parallel, the development of MEMS liquid chromatography (LC) provides a new venue to measure circulating oxidized low-density lipoprotein (ox-LDL) particles as a lab-on-a chip platform. Nanowire-based field effect transistors further pave the way for a high throughput approach to analyze the LDL redox state. Integration of MEMS with oxidative biology is synergistic in assessing vascular oxidative stress. The MEMS LC provides an emerging lab-on-a-chip platform for ox-LDL analysis. In this context, this chapter has integrated expertise from the fields of vascular biology and oxidative biology to address the dynamics of inflammatory responses.

Original languageEnglish (US)
Pages (from-to)111-150
Number of pages40
JournalMethods in Enzymology
Volume441
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Microtechnology
Nanotechnology
Oxidative stress
Endothelial cells
MEMS
Blood Vessels
Shear stress
Oxidative Stress
Endothelial Cells
NADPH Oxidase
Liquid Chromatography
Lab-on-a-chip
Fluids
Monitoring
Liquid chromatography
Nanowires
Systems Integration
Phagocytes
Vascular Smooth Muscle
Nitric Oxide Synthase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Chapter 7 Monitoring Oxidative Stress in Vascular Endothelial Cells in Response to Fluid Shear Stress : From Biochemical Analyses to Micro- and Nanotechnologies. / Rouhanizadeh, Mahsa; Takabe, Wakako; Ai, Lisong; Yu, Hongyu; Hsiai, Tzung.

In: Methods in Enzymology, Vol. 441, 2008, p. 111-150.

Research output: Contribution to journalArticle

@article{0610787f286b468783bac5c769f3723b,
title = "Chapter 7 Monitoring Oxidative Stress in Vascular Endothelial Cells in Response to Fluid Shear Stress: From Biochemical Analyses to Micro- and Nanotechnologies",
abstract = "Hemodynamics, specifically, fluid shear stress, modulates the focal nature of atherosclerosis. Shear stress induces vascular oxidative stress via the activation of membrane-bound NADPH oxidases present in vascular smooth muscle cells, fibroblasts, and phagocytic mononuclear cells. Shear stress acting on the endothelial cells at arterial bifurcations or branching points regulates both NADPH oxidase and nitric oxide (NO) synthase activities. The former is considered a major source of oxygen-centered radicals (i.e., superoxide anion [O2· -]) that give rise to oxidative stress; the latter is a source of nitrogen-centered radicals (i.e., nitric oxide [NO]) that give rise to nitrative/nitrosative stress. In addition to conventional biochemical analyses, the emerging microelectromechanical systems (MEMS) provide spatial and temporal resolutions to investigate the mechanisms whereby the characteristics of shear stress regulate the biological activities of endothelial cells at the complicated arterial geometry. In parallel, the development of MEMS liquid chromatography (LC) provides a new venue to measure circulating oxidized low-density lipoprotein (ox-LDL) particles as a lab-on-a chip platform. Nanowire-based field effect transistors further pave the way for a high throughput approach to analyze the LDL redox state. Integration of MEMS with oxidative biology is synergistic in assessing vascular oxidative stress. The MEMS LC provides an emerging lab-on-a-chip platform for ox-LDL analysis. In this context, this chapter has integrated expertise from the fields of vascular biology and oxidative biology to address the dynamics of inflammatory responses.",
author = "Mahsa Rouhanizadeh and Wakako Takabe and Lisong Ai and Hongyu Yu and Tzung Hsiai",
year = "2008",
doi = "10.1016/S0076-6879(08)01207-X",
language = "English (US)",
volume = "441",
pages = "111--150",
journal = "Methods",
issn = "1046-2023",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Chapter 7 Monitoring Oxidative Stress in Vascular Endothelial Cells in Response to Fluid Shear Stress

T2 - From Biochemical Analyses to Micro- and Nanotechnologies

AU - Rouhanizadeh, Mahsa

AU - Takabe, Wakako

AU - Ai, Lisong

AU - Yu, Hongyu

AU - Hsiai, Tzung

PY - 2008

Y1 - 2008

N2 - Hemodynamics, specifically, fluid shear stress, modulates the focal nature of atherosclerosis. Shear stress induces vascular oxidative stress via the activation of membrane-bound NADPH oxidases present in vascular smooth muscle cells, fibroblasts, and phagocytic mononuclear cells. Shear stress acting on the endothelial cells at arterial bifurcations or branching points regulates both NADPH oxidase and nitric oxide (NO) synthase activities. The former is considered a major source of oxygen-centered radicals (i.e., superoxide anion [O2· -]) that give rise to oxidative stress; the latter is a source of nitrogen-centered radicals (i.e., nitric oxide [NO]) that give rise to nitrative/nitrosative stress. In addition to conventional biochemical analyses, the emerging microelectromechanical systems (MEMS) provide spatial and temporal resolutions to investigate the mechanisms whereby the characteristics of shear stress regulate the biological activities of endothelial cells at the complicated arterial geometry. In parallel, the development of MEMS liquid chromatography (LC) provides a new venue to measure circulating oxidized low-density lipoprotein (ox-LDL) particles as a lab-on-a chip platform. Nanowire-based field effect transistors further pave the way for a high throughput approach to analyze the LDL redox state. Integration of MEMS with oxidative biology is synergistic in assessing vascular oxidative stress. The MEMS LC provides an emerging lab-on-a-chip platform for ox-LDL analysis. In this context, this chapter has integrated expertise from the fields of vascular biology and oxidative biology to address the dynamics of inflammatory responses.

AB - Hemodynamics, specifically, fluid shear stress, modulates the focal nature of atherosclerosis. Shear stress induces vascular oxidative stress via the activation of membrane-bound NADPH oxidases present in vascular smooth muscle cells, fibroblasts, and phagocytic mononuclear cells. Shear stress acting on the endothelial cells at arterial bifurcations or branching points regulates both NADPH oxidase and nitric oxide (NO) synthase activities. The former is considered a major source of oxygen-centered radicals (i.e., superoxide anion [O2· -]) that give rise to oxidative stress; the latter is a source of nitrogen-centered radicals (i.e., nitric oxide [NO]) that give rise to nitrative/nitrosative stress. In addition to conventional biochemical analyses, the emerging microelectromechanical systems (MEMS) provide spatial and temporal resolutions to investigate the mechanisms whereby the characteristics of shear stress regulate the biological activities of endothelial cells at the complicated arterial geometry. In parallel, the development of MEMS liquid chromatography (LC) provides a new venue to measure circulating oxidized low-density lipoprotein (ox-LDL) particles as a lab-on-a chip platform. Nanowire-based field effect transistors further pave the way for a high throughput approach to analyze the LDL redox state. Integration of MEMS with oxidative biology is synergistic in assessing vascular oxidative stress. The MEMS LC provides an emerging lab-on-a-chip platform for ox-LDL analysis. In this context, this chapter has integrated expertise from the fields of vascular biology and oxidative biology to address the dynamics of inflammatory responses.

UR - http://www.scopus.com/inward/record.url?scp=52249106000&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=52249106000&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(08)01207-X

DO - 10.1016/S0076-6879(08)01207-X

M3 - Article

VL - 441

SP - 111

EP - 150

JO - Methods

JF - Methods

SN - 1046-2023

ER -