@inbook{0fb635e2dd9241afb95ed73cedd51ff9,
title = "CHAPTER 17: High Viscosity Microstream Sample Delivery for Serial Femtosecond Crystallography",
abstract = "Serial femtosecond crystallography at an X-ray free electron laser (XFEL) provides a new method to solve protein structures from micrometer-sized crystals at room temperature with minimal radiation damage. Fast replenishment of the sample is needed due to the high repetition rate of the XFEL, and liquid injection methods have been used extensively. Delivery of microcrystals embedded in a high viscosity microstream provides for a considerable reduction in sample consumption compared to low viscosity liquid jets. This method allows the use of membrane protein crystals grown in lipidic cubic phase, as well as crystals mixed in other high viscosity matrixes.",
author = "Uwe Weierstall",
note = "Funding Information: Experiments were carried out at the Linac Coherent Light Source, a national user facility operated by Stanford University on behalf of the U.S. Department of Energy (DOE), Office of Basic Energy Sciences. we acknowledge support from the National Science Foundation (awards MCB 1021557 and MCB 1120997) and its BioXFEL Science and Technology Center (NSF 1231306). Special thanks to Claudiu Stan (pULSE Institute) and Sebasti{\'e}n Boutet (LCLS) for providing Figure 17.7 and information about the image collection technique used, and Vadim Cherezov (USC), who was the beamtime pI when the high speed images where recorded. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2017.",
year = "2017",
doi = "10.1039/9781782624097-00337",
language = "English (US)",
series = "RSC Energy and Environment Series",
publisher = "Royal Society of Chemistry",
number = "18",
pages = "337--347",
editor = "Uwe Bergmann and Yachandra, {Vittal K.} and Junko Yano",
booktitle = "Thermoelectric Materials and Devices",
edition = "18",
}