CHAPTER 17: High Viscosity Microstream Sample Delivery for Serial Femtosecond Crystallography

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Serial femtosecond crystallography at an X-ray free electron laser (XFEL) provides a new method to solve protein structures from micrometer-sized crystals at room temperature with minimal radiation damage. Fast replenishment of the sample is needed due to the high repetition rate of the XFEL, and liquid injection methods have been used extensively. Delivery of microcrystals embedded in a high viscosity microstream provides for a considerable reduction in sample consumption compared to low viscosity liquid jets. This method allows the use of membrane protein crystals grown in lipidic cubic phase, as well as crystals mixed in other high viscosity matrixes.

Original languageEnglish (US)
Title of host publicationThermoelectric Materials and Devices
EditorsUwe Bergmann, Vittal K. Yachandra, Junko Yano
PublisherRoyal Society of Chemistry
Pages337-347
Number of pages11
Edition18
ISBN (Electronic)9781782623236, 9781849731003
DOIs
StatePublished - Jan 1 2017

Publication series

NameRSC Energy and Environment Series
Number18
Volume2017-January
ISSN (Print)2044-0774
ISSN (Electronic)2044-0782

ASJC Scopus subject areas

  • Global and Planetary Change
  • Environmental Chemistry
  • Energy(all)

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  • Cite this

    Weierstall, U. (2017). CHAPTER 17: High Viscosity Microstream Sample Delivery for Serial Femtosecond Crystallography. In U. Bergmann, V. K. Yachandra, & J. Yano (Eds.), Thermoelectric Materials and Devices (18 ed., pp. 337-347). (RSC Energy and Environment Series; Vol. 2017-January, No. 18). Royal Society of Chemistry. https://doi.org/10.1039/9781782624097-00337