Chapter 10 M-T7. Measuring Chemokine-Modulating Activity

Mee Y. Bartee, Erbin Dai, Liying Liu, Ganesh Munuswamy-Ramanujam, Colin Macaulay, Dana McIvor, Grant McFadden, Alexandra R. Lucas

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Chemokines are important for activation of a host of cellular immune and inflammatory responses including cell signaling, activation, and communication. M-T7, a myxoma virus protein, inhibits the activity of chemokines by direct binding to chemokines and/or with glycosaminoglycans (GAGs). To study the effects of this chemokine-modulating protein (CMP), we use a variety of in vitro and in vivo techniques to evaluate M-T7 inhibition of inflammatory cells. To quickly analyze the effects of M-T7, changes in cell adhesion and membrane fluidity are measured as well as cell migration in mouse ascites. For more physiological analyses, an aortic transplant model in rodents is used to assess change in inflammatory cell infiltrates and vascular plaque growth (rejection). Utilization of the combination of these in vitro and in vivo techniques allows for a more complete study of the chemokine-modulating activity of M-T7, and can be used to study other immune and inflammation-modulating proteins.

Original languageEnglish (US)
Title of host publicationChemokines, Part A
EditorsJohn Abelson, Melvin Simon
Pages209-228
Number of pages20
EditionA
DOIs
StatePublished - May 14 2009
Externally publishedYes

Publication series

NameMethods in Enzymology
NumberA
Volume460
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Bartee, M. Y., Dai, E., Liu, L., Munuswamy-Ramanujam, G., Macaulay, C., McIvor, D., McFadden, G., & Lucas, A. R. (2009). Chapter 10 M-T7. Measuring Chemokine-Modulating Activity. In J. Abelson, & M. Simon (Eds.), Chemokines, Part A (A ed., pp. 209-228). (Methods in Enzymology; Vol. 460, No. A). https://doi.org/10.1016/S0076-6879(09)05210-0