TY - JOUR
T1 - Changes in susceptibility of normal cells to agglutination by plant lectins following modification of cell coat material
AU - Poste, G.
N1 - Funding Information:
The presente xperimentsh ave demonstrated ceptible to lectin agglutination throughout that the agglutinationo f cells by ConA and the cell cycle.I n view of the presentf indings WGA is not a specificp ropertyo f cellst rans-with vitamin A and anticellulars erum,i t is formed by oncogenicv iruses. These results temptingt o speculatet hat the modification confirm the recento bservationso f Zarling & of the cell surface by lysosomal enzymes Tevethia [48] who found that infection of might be an important factor in producing rabbit kidney cells with vaccinia virus in-the surfacec hangesr esponsiblefo r the agglu-creased significantly their susceptibility to tination of the cell by lectins.T hus, in normal cells enzymicm odificationo f the cell periph-facec omponentsw hich are presentin normal ery would be confinedt o the,mitotic period cells in a differenti naccessiblefo rm [see1 1, while in transformedc ells the processw ould 16,2 21o r, in somec asesi,n a form expressed be continuous. only in embryonicd evelopmen[t2 0, 24, 291. The importance of lysosomal-induced It would be of interest thereforet o see if changesin the cell peripherya s a regulatory modificationo f the cell coat in normal cells factor in cell growth has beenp roposedp reusing the treatmentsd escribedh ere would viously by several authors, notably Weiss result in the ‘exposure’ of other allegedly [47]a nd Dingle [15].I t is pertinentt herefore ‘tumour-specific’a nd ‘embryonic’ cell sur-to note that the extracellularr eleaseo f lyso-facec omponentsT. his possibilityi s currently somal enzymesis a well documentedfe ature under investigation. of mitosis in normal cells [see1 1.S imilarly, increased levels of lysosomal glycosidases agglutinin preparation. I thank Mr G. Terry for have been found in polyoma virus-trans-This work was supported by grantsf rom the Med- formed cells comparedw ith normal cells [7, paign and the National Fund for Research into ical Research Council, the Cancer Research Cam- 81 and Rubin [42] has shown recentlyt hat Crippling Diseases (Polio Research Fund). Rous sarcomav irus-transformedc hick cells
PY - 1972/8
Y1 - 1972/8
N2 - Infection of normal chick embryo, baby hamster kidney and HEp-2 cells with the nononcogenic viruses, Newcastle disease virus and Herpes simplex virus, was found to increase their susceptibility to agglutination by the plant lectins, concanavalin A and wheat germ agglutinin. Similar results were obtained following treatment of normal HEp-2 cells with anticellular serum and high concentrations of vitamin A alcohol. The agglutination reaction with concanavalin A and wheat germ agglutinin was inhibited specifically by α-methyl-d-glucopyranoside and N-acetyl-glucosamine, respectively. A temporal correlation was demonstrated between changes in cell agglutination behaviour and digestion of the cell coat material. The relationship of changes occurring in the cell coat to the observed alterations in cell agglutination behaviour was discussed with reference to the role of enzymic modification of the cell periphery as a factor in cell growth regulation.
AB - Infection of normal chick embryo, baby hamster kidney and HEp-2 cells with the nononcogenic viruses, Newcastle disease virus and Herpes simplex virus, was found to increase their susceptibility to agglutination by the plant lectins, concanavalin A and wheat germ agglutinin. Similar results were obtained following treatment of normal HEp-2 cells with anticellular serum and high concentrations of vitamin A alcohol. The agglutination reaction with concanavalin A and wheat germ agglutinin was inhibited specifically by α-methyl-d-glucopyranoside and N-acetyl-glucosamine, respectively. A temporal correlation was demonstrated between changes in cell agglutination behaviour and digestion of the cell coat material. The relationship of changes occurring in the cell coat to the observed alterations in cell agglutination behaviour was discussed with reference to the role of enzymic modification of the cell periphery as a factor in cell growth regulation.
UR - http://www.scopus.com/inward/record.url?scp=0015383289&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0015383289&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(72)90054-7
DO - 10.1016/0014-4827(72)90054-7
M3 - Article
C2 - 4341006
AN - SCOPUS:0015383289
SN - 0014-4827
VL - 73
SP - 319
EP - 328
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -