Challenges in creating a vaccine to prevent hepatitis E

Bryan J. Maloney, Naokazu Takeda, Yuriko Suzaki, Yasushi Ami, Tian Cheng Li, Tatsuo Miyamura, Charles J. Arntzen, Hugh Mason

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Recombinant hepatitis E virus capsid protein (HEV CP) assembles orally immunogenic virus-like particles (VLP) when expressed in an insect cell system. We used plant expression cassettes, pHEV101 and pHEV110, for transformation of potato to express HEV CP, and 10 independent transgenic lines of HEV101 and 6 lines of HEV110 were obtained. ELISA for HEV CP was performed on tuber extracts. Accumulation of HEV CP in tubers varied from about 5 to 30 μg/g fresh tuber depending on the transgenic plant line. We further compared the expression levels with the yield of tubers for each line. Tuber yield varied less than expression levels, and ranged from about 600 to 1000 g per pot. Although Western blot showed that apparently intact HEV CP accumulated, we observed very limited assembly of virus-like particles in potato tubers. Oral immunization of mice with transgenic potatoes failed to elicit detectable anti-CP antibody response in serum, suggesting that VLP assembly is a key factor in orally delivered HEV CP vaccines.

Original languageEnglish (US)
Pages (from-to)1870-1874
Number of pages5
JournalVaccine
Volume23
Issue number15 SPEC. ISS.
DOIs
StatePublished - Mar 7 2005

Keywords

  • HEV
  • Hepatitis E
  • Transgenic potato
  • VLP

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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  • Cite this

    Maloney, B. J., Takeda, N., Suzaki, Y., Ami, Y., Li, T. C., Miyamura, T., Arntzen, C. J., & Mason, H. (2005). Challenges in creating a vaccine to prevent hepatitis E. Vaccine, 23(15 SPEC. ISS.), 1870-1874. https://doi.org/10.1016/j.vaccine.2004.11.020