Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings

R. Mishra, R. Alam, D. McAuley, T. Bharaj, D. Kinahan, C. Nwankire, Karen Anderson, J. Ducrée

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Infections of high-risk strains of human papilloma virus (HPV16-E7) have been directly linked with cervical carcinogenesis [1]. Despite pervasive HPV testing and vaccinations, resource-poor regions continue to be affected. We present here a fully automated, bead-based Rapid Antigenic Protein In-situ Display (RAPID) Enzyme Linked Immunosorbent Assay (ELISA) for the detection of the onco-proteins of HPV16 E7 [1]. Advanced centrifugal flow control is implemented by a novel router based on lipophilic membranes in an event-trigger valving architecture [2]. By simply replacing the charge of RAPID-functionalized beads in the incubation chamber, the system may be customized for the detection of other biomarkers.

    Original languageEnglish (US)
    Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
    PublisherChemical and Biological Microsystems Society
    Pages844-845
    Number of pages2
    ISBN (Electronic)9780979806490
    StatePublished - 2016
    Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
    Duration: Oct 9 2016Oct 13 2016

    Other

    Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
    CountryIreland
    CityDublin
    Period10/9/1610/13/16

    Fingerprint

    Automation
    Proteins
    Biomarkers
    Viruses
    Routers
    Flow control
    Assays
    Enzymes
    Display devices
    Membranes
    Testing
    Immunosorbents

    Keywords

    • Centrifugal microfluidics
    • Cervical cancer
    • Lab-on-a-Disc (LoaD)
    • Lipophilic membranes
    • RAPID ELISA
    • Routing

    ASJC Scopus subject areas

    • Control and Systems Engineering

    Cite this

    Mishra, R., Alam, R., McAuley, D., Bharaj, T., Kinahan, D., Nwankire, C., ... Ducrée, J. (2016). Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 844-845). Chemical and Biological Microsystems Society.

    Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings. / Mishra, R.; Alam, R.; McAuley, D.; Bharaj, T.; Kinahan, D.; Nwankire, C.; Anderson, Karen; Ducrée, J.

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 844-845.

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Mishra, R, Alam, R, McAuley, D, Bharaj, T, Kinahan, D, Nwankire, C, Anderson, K & Ducrée, J 2016, Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, pp. 844-845, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 10/9/16.
    Mishra R, Alam R, McAuley D, Bharaj T, Kinahan D, Nwankire C et al. Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 844-845
    Mishra, R. ; Alam, R. ; McAuley, D. ; Bharaj, T. ; Kinahan, D. ; Nwankire, C. ; Anderson, Karen ; Ducrée, J. / Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 844-845
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