Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings

R. Mishra, R. Alam, D. McAuley, T. Bharaj, D. Kinahan, C. Nwankire, Karen Anderson, J. Ducrée

Abstract

Infections of high-risk strains of human papilloma virus (HPV16-E7) have been directly linked with cervical carcinogenesis [1]. Despite pervasive HPV testing and vaccinations, resource-poor regions continue to be affected. We present here a fully automated, bead-based Rapid Antigenic Protein In-situ Display (RAPID) Enzyme Linked Immunosorbent Assay (ELISA) for the detection of the onco-proteins of HPV16 E7 [1]. Advanced centrifugal flow control is implemented by a novel router based on lipophilic membranes in an event-trigger valving architecture [2]. By simply replacing the charge of RAPID-functionalized beads in the incubation chamber, the system may be customized for the detection of other biomarkers.

Original languageEnglish (US)
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages844-845
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: Oct 9 2016Oct 13 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period10/9/1610/13/16

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Mishra, R., Alam, R., McAuley, D., Bharaj, T., Kinahan, D., Nwankire, C., ... Ducrée, J. (2016). Centrifugal automation of a highly customizable rapid-elisa test towards detection of cervical cancer in point-of-care settings. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 844-845). Chemical and Biological Microsystems Society.