Cementum annulation and age determination in Homo sapiens. I. Tooth variability and observer error

D. K. Charles, K. Condon, J. M. Cheverud, Jane Buikstra

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

In order to test the feasibility of cementum annulations to estimate age in humans, observer error and tooth variability in cementum ring counts were evaluated in a sample of 42 mandibular canine and first premolar pairs. Additionally, two sectioning techniques were evaluated. Demineralized thin sections (7 μm) stained with hematoxylin are the preferred technique since their age related variance is greater than 75% for all tooth types examined. In contrast, less than 50% of the total variance was accounted for among individuals when mineralized sections (80 μm) stained with alizarin red were used. Intertooth variability in ring counts of demineralized sections was large between canines and premolars (43%). Premolars provide counts with lower interobserver error and are the preferred tooth. In an expanded sample (N = 51) of demineralized premolars, intraobserver and interobserver error accounted for 2% and 5% of the total variance, respectively. Evaluation of several experimental designs showed that increasing the number of slides per tooth has the greatest effect on reducing variance followed by increasing the number of observers. Increasing the number of observations has little effect. Cementum ring counts are measurable to a highly repeatable extent and provide a level of repeatability greater than that reported for the pubic symphysis and auricular surface aging techniques.

Original languageEnglish (US)
Pages (from-to)311-320
Number of pages10
JournalAmerican Journal of Physical Anthropology
Volume71
Issue number3
StatePublished - 1986
Externally publishedYes

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Dental Cementum
age determination
Bicuspid
Tooth
teeth
human error
Canidae
Pubic Symphysis
alizarin
dogs
Hematoxylin
evaluation
repeatability
Research Design
experimental design
methodology
sampling
Homo sapiens
testing

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anthropology

Cite this

Cementum annulation and age determination in Homo sapiens. I. Tooth variability and observer error. / Charles, D. K.; Condon, K.; Cheverud, J. M.; Buikstra, Jane.

In: American Journal of Physical Anthropology, Vol. 71, No. 3, 1986, p. 311-320.

Research output: Contribution to journalArticle

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