Cellular and cytokine responses to Salmonella enterica serotype typhi proteins in patients with typhoid fever in Bangladesh

Md Saruar Bhuiyan, Md Abu Sayeed, Farhana Khanam, Daniel T. Leung, Taufiqur Rahman Bhuiyan, Alaullah Sheikh, Umme Salma, Regina C. LaRocque, Jason B. Harris, Marcin Pacek, Stephen B. Calderwood, Joshua LaBaer, Edward T. Ryan, Firdausi Qadri, Richelle C. Charles

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We assessed interferon-gamma (IFN-γ) responses via enzyme-linked immunosorbent spot (ELISPOT) to a number of S. Typhi antigens in samples from humans with S. Typhi bacteremia and typhoid fever in Bangladesh. Compared with responses in healthy endemic zone controls, there were significantly increased IFN-γ responses at the time of clinical presentation (acute phase) and at convalescence 14-28 days later. The majority (80-90%) of IFN-γ expressing T cells were CD4+. We observed a significant increase in interleukin-17 (IL-17) positive CD4 + T cells at convalescent versus acute stage of infection using an intracellular cytokine staining assay. We also found that stimulated peripheral blood mononuclear cells (PBMCs) produced significantly increased levels of a number of cytokines at the convalescent versus acute phase of infection, including IFN-γ, MIP-1β, sCD40L, TNF-β, IL-13, and IL-9. These results suggest that S. Typhi antigens induce a predominantly Th1 response, but that elevations in other cytokines may be modulatory.

Original languageEnglish (US)
Pages (from-to)1024-1030
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume90
Issue number6
DOIs
StatePublished - 2014

Fingerprint

Salmonella enterica
Bangladesh
Typhoid Fever
Interferon-gamma
Cytokines
Proteins
Interleukin-9
T-Lymphocytes
Antigens
Immunosorbents
Interleukin-13
Interleukin-17
Bacteremia
Infection
Blood Cells
Staining and Labeling
Serogroup
Enzymes

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Cellular and cytokine responses to Salmonella enterica serotype typhi proteins in patients with typhoid fever in Bangladesh. / Bhuiyan, Md Saruar; Sayeed, Md Abu; Khanam, Farhana; Leung, Daniel T.; Bhuiyan, Taufiqur Rahman; Sheikh, Alaullah; Salma, Umme; LaRocque, Regina C.; Harris, Jason B.; Pacek, Marcin; Calderwood, Stephen B.; LaBaer, Joshua; Ryan, Edward T.; Qadri, Firdausi; Charles, Richelle C.

In: American Journal of Tropical Medicine and Hygiene, Vol. 90, No. 6, 2014, p. 1024-1030.

Research output: Contribution to journalArticle

Bhuiyan, MS, Sayeed, MA, Khanam, F, Leung, DT, Bhuiyan, TR, Sheikh, A, Salma, U, LaRocque, RC, Harris, JB, Pacek, M, Calderwood, SB, LaBaer, J, Ryan, ET, Qadri, F & Charles, RC 2014, 'Cellular and cytokine responses to Salmonella enterica serotype typhi proteins in patients with typhoid fever in Bangladesh', American Journal of Tropical Medicine and Hygiene, vol. 90, no. 6, pp. 1024-1030. https://doi.org/10.4269/ajtmh.13-0261
Bhuiyan, Md Saruar ; Sayeed, Md Abu ; Khanam, Farhana ; Leung, Daniel T. ; Bhuiyan, Taufiqur Rahman ; Sheikh, Alaullah ; Salma, Umme ; LaRocque, Regina C. ; Harris, Jason B. ; Pacek, Marcin ; Calderwood, Stephen B. ; LaBaer, Joshua ; Ryan, Edward T. ; Qadri, Firdausi ; Charles, Richelle C. / Cellular and cytokine responses to Salmonella enterica serotype typhi proteins in patients with typhoid fever in Bangladesh. In: American Journal of Tropical Medicine and Hygiene. 2014 ; Vol. 90, No. 6. pp. 1024-1030.
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AU - Calderwood, Stephen B.

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