Cellular and cytokine responses to Salmonella enterica serotype typhi proteins in patients with typhoid fever in Bangladesh

Md Saruar Bhuiyan, Md Abu Sayeed, Farhana Khanam, Daniel T. Leung, Taufiqur Rahman Bhuiyan, Alaullah Sheikh, Umme Salma, Regina C. LaRocque, Jason B. Harris, Marcin Pacek, Stephen B. Calderwood, Joshua LaBaer, Edward T. Ryan, Firdausi Qadri, Richelle C. Charles

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

We assessed interferon-gamma (IFN-γ) responses via enzyme-linked immunosorbent spot (ELISPOT) to a number of S. Typhi antigens in samples from humans with S. Typhi bacteremia and typhoid fever in Bangladesh. Compared with responses in healthy endemic zone controls, there were significantly increased IFN-γ responses at the time of clinical presentation (acute phase) and at convalescence 14-28 days later. The majority (80-90%) of IFN-γ expressing T cells were CD4+. We observed a significant increase in interleukin-17 (IL-17) positive CD4 + T cells at convalescent versus acute stage of infection using an intracellular cytokine staining assay. We also found that stimulated peripheral blood mononuclear cells (PBMCs) produced significantly increased levels of a number of cytokines at the convalescent versus acute phase of infection, including IFN-γ, MIP-1β, sCD40L, TNF-β, IL-13, and IL-9. These results suggest that S. Typhi antigens induce a predominantly Th1 response, but that elevations in other cytokines may be modulatory.

Original languageEnglish (US)
Pages (from-to)1024-1030
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume90
Issue number6
DOIs
StatePublished - Jun 2014

ASJC Scopus subject areas

  • Parasitology
  • Virology
  • Infectious Diseases

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