TY - JOUR
T1 - Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform
AU - Romani, Patrizia
AU - Bernardi, Fabio
AU - Hackney, Jennifer
AU - Dobens, Leonard
AU - Gargiulo, Giuseppe
AU - Cavaliere, Valeria
PY - 2009/1
Y1 - 2009/1
N2 - Proper assembly and maintenance of epithelia are critical for normal development and homeostasis. Here, using the Drosophila ovary as a model, we identify a role for the B1 isoform of the ecdysone receptor (EcR-B1) in this process. We performed a reverse genetic analysis of EcR-B1 function during oogenesis and demonstrate that silencing of this receptor isoform causes loss of integrity and multilayering of the follicular epithelium. We show that multilayered follicle cells lack proper cell polarity with altered distribution of apical and basolateral cell polarity markers including atypical-protein kinase C (aPKC), Discs-large (Dlg), and Scribble (Scrib) and aberrant accumulation of adherens junctions and F-actin cytoskeleton. We find that the EcR-B1 isoform is required for proper follicle cell polarity both during early stages of oogenesis, when follicle cells undergo the mitotic cell cycle, and at midoogenesis when these cells stop dividing and undergo several endocycles. In addition, we show that the EcR-B1 isoform is required during early oogenesis for follicle cell survival and that disruption of its function causes apoptotic cell death induced by caspase.
AB - Proper assembly and maintenance of epithelia are critical for normal development and homeostasis. Here, using the Drosophila ovary as a model, we identify a role for the B1 isoform of the ecdysone receptor (EcR-B1) in this process. We performed a reverse genetic analysis of EcR-B1 function during oogenesis and demonstrate that silencing of this receptor isoform causes loss of integrity and multilayering of the follicular epithelium. We show that multilayered follicle cells lack proper cell polarity with altered distribution of apical and basolateral cell polarity markers including atypical-protein kinase C (aPKC), Discs-large (Dlg), and Scribble (Scrib) and aberrant accumulation of adherens junctions and F-actin cytoskeleton. We find that the EcR-B1 isoform is required for proper follicle cell polarity both during early stages of oogenesis, when follicle cells undergo the mitotic cell cycle, and at midoogenesis when these cells stop dividing and undergo several endocycles. In addition, we show that the EcR-B1 isoform is required during early oogenesis for follicle cell survival and that disruption of its function causes apoptotic cell death induced by caspase.
UR - http://www.scopus.com/inward/record.url?scp=61549085609&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61549085609&partnerID=8YFLogxK
U2 - 10.1534/genetics.108.096008
DO - 10.1534/genetics.108.096008
M3 - Article
C2 - 19015542
AN - SCOPUS:61549085609
SN - 0016-6731
VL - 181
SP - 165
EP - 175
JO - Genetics
JF - Genetics
IS - 1
ER -