Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform

Patrizia Romani, Fabio Bernardi, Jennifer Hackney Price, Leonard Dobens, Giuseppe Gargiulo, Valeria Cavaliere

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Proper assembly and maintenance of epithelia are critical for normal development and homeostasis. Here, using the Drosophila ovary as a model, we identify a role for the B1 isoform of the ecdysone receptor (EcR-B1) in this process. We performed a reverse genetic analysis of EcR-B1 function during oogenesis and demonstrate that silencing of this receptor isoform causes loss of integrity and multilayering of the follicular epithelium. We show that multilayered follicle cells lack proper cell polarity with altered distribution of apical and basolateral cell polarity markers including atypical-protein kinase C (aPKC), Discs-large (Dlg), and Scribble (Scrib) and aberrant accumulation of adherens junctions and F-actin cytoskeleton. We find that the EcR-B1 isoform is required for proper follicle cell polarity both during early stages of oogenesis, when follicle cells undergo the mitotic cell cycle, and at midoogenesis when these cells stop dividing and undergo several endocycles. In addition, we show that the EcR-B1 isoform is required during early oogenesis for follicle cell survival and that disruption of its function causes apoptotic cell death induced by caspase.

Original languageEnglish (US)
Pages (from-to)165-175
Number of pages11
JournalGenetics
Volume181
Issue number1
DOIs
StatePublished - Jan 2009
Externally publishedYes

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Cell Polarity
Oogenesis
Drosophila
Cell Survival
Protein Isoforms
Epithelium
Adherens Junctions
Reverse Genetics
Caspases
Actin Cytoskeleton
Actins
Ovary
Cell Cycle
Homeostasis
Cell Death
Maintenance
ecdysone receptor

ASJC Scopus subject areas

  • Genetics

Cite this

Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform. / Romani, Patrizia; Bernardi, Fabio; Hackney Price, Jennifer; Dobens, Leonard; Gargiulo, Giuseppe; Cavaliere, Valeria.

In: Genetics, Vol. 181, No. 1, 01.2009, p. 165-175.

Research output: Contribution to journalArticle

Romani, Patrizia ; Bernardi, Fabio ; Hackney Price, Jennifer ; Dobens, Leonard ; Gargiulo, Giuseppe ; Cavaliere, Valeria. / Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform. In: Genetics. 2009 ; Vol. 181, No. 1. pp. 165-175.
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