Cell distribution profiles in three-dimensional scaffolds with inverted-colloidal-crystal geometry: Modeling and experimental investigations

Sachin Shanbhag, Shaopeng Wang, Nicholas A. Kotov

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Limited ingrowth of stromal cells is observed when a three-dimensionally ordered scaffold possessing inverted-colloidal-crystal geometry is used to culture adherent cells. In this work, a computational model explaining, as well as predicting, experimental cell distributions is developed. It incorporates a modified Contois cell-growth model that includes the effects of nutrient saturation, competitive product inhibition, and cell-contact inhibition to describe the scaffold-cell system. Our results agree with the hypothesis that the rapid growth of cells on the surface of the scaffold depletes the nutrient supply to the core, resulting in the preferential growth on the exterior of the scaffold. When the cells are cultured in a scaffold subjected to a uniform velocity field, they penetrate to a greater extent into the scaffold core. Alternative seeding and culture strategies are suggested and evaluated.

Original languageEnglish (US)
Pages (from-to)1208-1214
Number of pages7
JournalSmall
Volume1
Issue number12
DOIs
StatePublished - Dec 1 2005
Externally publishedYes

Keywords

  • Cell growth
  • Colloids
  • Diffusion
  • Modeling
  • Scaffolds

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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