Abstract

Cationic surfactants can be used to improve extraction of high-value products from microalgae, but the best way to apply cationic surfactants has not been established. We evaluated the impacts of contact time and concentration for two cationic surfactants – dodecyltrimethylammonium bromide (DTAB) and hexadecyltrimethylammonium bromide (CTAB) – on extraction of lipids and nonpolar pigments, carotenoids and chlorophyll a, from Synechocystis sp. PCC 6803. For the most dose-effective concentration, pigment extraction was better with the longer alkyl-chain length, i.e., CTAB > DTAB, because the longer alkyl chain of CTAB increased cell membrane permeability, causing cell lysis that allowed solvent-pigment contact. A CTAB dose of 25 mM and treatment for 5 h gave the highest pigment extraction, nearly 30 and 12 mg/g dried biomass for chlorophyll a and carotenoids, respectively. In contrast, lipid recovery was better with the shorter alkyl-chain length, DTAB > CTAB. DTAB disrupted the cells less, which allowed the solvent to access more lipids held in the cells. Thus, the selection of surfactant and dosing strategy depends on which products are targeted. With moderate cell disruption, a moderate dose of CTAB (10 mM) was effective for recovering pigments and lipids simultaneously.

Original languageEnglish (US)
Pages (from-to)250-255
Number of pages6
JournalAlgal Research
Volume34
DOIs
StatePublished - Sep 1 2018

Fingerprint

biobased products
Synechocystis sp. PCC 6803
bromides
pigments
lipids
cells
carotenoids
dosage
high-value products
chlorophyll
membrane permeability
microalgae
surfactants
cationic surfactants
biomass

Keywords

  • Cationic surfactants
  • Cyanobacteria
  • Flow cytometry
  • Harvesting and extraction
  • Synechocystis

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Cell disruption by cationic surfactants affects bioproduct recovery from Synechocystis sp. PCC 6803. / Lai, Yen Jung Sean; Zhou, Yun; Eustance, Everett; Straka, Levi; Wang, Zhaocheng; Rittmann, Bruce.

In: Algal Research, Vol. 34, 01.09.2018, p. 250-255.

Research output: Contribution to journalArticle

Lai, Yen Jung Sean ; Zhou, Yun ; Eustance, Everett ; Straka, Levi ; Wang, Zhaocheng ; Rittmann, Bruce. / Cell disruption by cationic surfactants affects bioproduct recovery from Synechocystis sp. PCC 6803. In: Algal Research. 2018 ; Vol. 34. pp. 250-255.
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