Abstract
Cationic surfactants can be used to improve extraction of high-value products from microalgae, but the best way to apply cationic surfactants has not been established. We evaluated the impacts of contact time and concentration for two cationic surfactants – dodecyltrimethylammonium bromide (DTAB) and hexadecyltrimethylammonium bromide (CTAB) – on extraction of lipids and nonpolar pigments, carotenoids and chlorophyll a, from Synechocystis sp. PCC 6803. For the most dose-effective concentration, pigment extraction was better with the longer alkyl-chain length, i.e., CTAB > DTAB, because the longer alkyl chain of CTAB increased cell membrane permeability, causing cell lysis that allowed solvent-pigment contact. A CTAB dose of 25 mM and treatment for 5 h gave the highest pigment extraction, nearly 30 and 12 mg/g dried biomass for chlorophyll a and carotenoids, respectively. In contrast, lipid recovery was better with the shorter alkyl-chain length, DTAB > CTAB. DTAB disrupted the cells less, which allowed the solvent to access more lipids held in the cells. Thus, the selection of surfactant and dosing strategy depends on which products are targeted. With moderate cell disruption, a moderate dose of CTAB (10 mM) was effective for recovering pigments and lipids simultaneously.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 250-255 |
| Number of pages | 6 |
| Journal | Algal Research |
| Volume | 34 |
| DOIs | |
| State | Published - Sep 1 2018 |
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Keywords
- Cationic surfactants
- Cyanobacteria
- Flow cytometry
- Harvesting and extraction
- Synechocystis
ASJC Scopus subject areas
- Agronomy and Crop Science
Cite this
Cell disruption by cationic surfactants affects bioproduct recovery from Synechocystis sp. PCC 6803. / Lai, Yen Jung Sean; Zhou, Yun; Eustance, Everett; Straka, Levi; Wang, Zhaocheng; Rittmann, Bruce.
In: Algal Research, Vol. 34, 01.09.2018, p. 250-255.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cell disruption by cationic surfactants affects bioproduct recovery from Synechocystis sp. PCC 6803
AU - Lai, Yen Jung Sean
AU - Zhou, Yun
AU - Eustance, Everett
AU - Straka, Levi
AU - Wang, Zhaocheng
AU - Rittmann, Bruce
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Cationic surfactants can be used to improve extraction of high-value products from microalgae, but the best way to apply cationic surfactants has not been established. We evaluated the impacts of contact time and concentration for two cationic surfactants – dodecyltrimethylammonium bromide (DTAB) and hexadecyltrimethylammonium bromide (CTAB) – on extraction of lipids and nonpolar pigments, carotenoids and chlorophyll a, from Synechocystis sp. PCC 6803. For the most dose-effective concentration, pigment extraction was better with the longer alkyl-chain length, i.e., CTAB > DTAB, because the longer alkyl chain of CTAB increased cell membrane permeability, causing cell lysis that allowed solvent-pigment contact. A CTAB dose of 25 mM and treatment for 5 h gave the highest pigment extraction, nearly 30 and 12 mg/g dried biomass for chlorophyll a and carotenoids, respectively. In contrast, lipid recovery was better with the shorter alkyl-chain length, DTAB > CTAB. DTAB disrupted the cells less, which allowed the solvent to access more lipids held in the cells. Thus, the selection of surfactant and dosing strategy depends on which products are targeted. With moderate cell disruption, a moderate dose of CTAB (10 mM) was effective for recovering pigments and lipids simultaneously.
AB - Cationic surfactants can be used to improve extraction of high-value products from microalgae, but the best way to apply cationic surfactants has not been established. We evaluated the impacts of contact time and concentration for two cationic surfactants – dodecyltrimethylammonium bromide (DTAB) and hexadecyltrimethylammonium bromide (CTAB) – on extraction of lipids and nonpolar pigments, carotenoids and chlorophyll a, from Synechocystis sp. PCC 6803. For the most dose-effective concentration, pigment extraction was better with the longer alkyl-chain length, i.e., CTAB > DTAB, because the longer alkyl chain of CTAB increased cell membrane permeability, causing cell lysis that allowed solvent-pigment contact. A CTAB dose of 25 mM and treatment for 5 h gave the highest pigment extraction, nearly 30 and 12 mg/g dried biomass for chlorophyll a and carotenoids, respectively. In contrast, lipid recovery was better with the shorter alkyl-chain length, DTAB > CTAB. DTAB disrupted the cells less, which allowed the solvent to access more lipids held in the cells. Thus, the selection of surfactant and dosing strategy depends on which products are targeted. With moderate cell disruption, a moderate dose of CTAB (10 mM) was effective for recovering pigments and lipids simultaneously.
KW - Cationic surfactants
KW - Cyanobacteria
KW - Flow cytometry
KW - Harvesting and extraction
KW - Synechocystis
UR - http://www.scopus.com/inward/record.url?scp=85051271645&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85051271645&partnerID=8YFLogxK
U2 - 10.1016/j.algal.2018.08.010
DO - 10.1016/j.algal.2018.08.010
M3 - Article
AN - SCOPUS:85051271645
VL - 34
SP - 250
EP - 255
JO - Algal Research
JF - Algal Research
SN - 2211-9264
ER -