Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of asp176, glu179 and glu180 in the enzyme from Aspergillus awamori

Engineering & Materials Science

• Maltose 100%
• Mutagenesis 87%
• Aspergillus 87%
• Hydrolysis 66%
• Enzymes 63%
• Activation energy 57%
• Binding energy 43%
• Substrates 38%
• Kinetic parameters 33%
• Substitution reactions 27%
• Labeling 26%
• Acids 25%

Chemical Compounds

• Maltoheptaose 87%
• Isomaltose 86%
• Maltose 65%
• Asn-Gln 46%
• Reaction Activation Energy 38%
• Hydrolysis 34%
• pKa 29%
• Binding Energy 24%
• Mutation 23%
• Transition State 22%
• Pharmacokinetics 20%
• Substitution Reaction 17%
• pH Value 11%
• Acid 9%

Medicine & Life Sciences

• Glucan 1,4-alpha-Glucosidase 86%
• Aspergillus 62%
• Mutagenesis 54%
• maltoheptaose 43%
• Isomaltose 40%
• Enzymes 30%
• Maltose 29%
• Hydrolysis 20%
• Catalytic Domain 9%
• Acids 8%
• Kinetics 8%
• Mutation 6%